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首页> 外文期刊>The journal of sexual medicine >Effects of Supraphysiological Testosterone Treatment and Orchiectomy on Ischemia/Reperfusion-Induced Bladder Dysfunction in Male Rabbits
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Effects of Supraphysiological Testosterone Treatment and Orchiectomy on Ischemia/Reperfusion-Induced Bladder Dysfunction in Male Rabbits

机译：超生理睾酮治疗和睾丸切除术对雄性兔缺血/再灌注所致膀胱功能障碍的影响

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Introduction: The roles of testosterone and orchiectomy on male bladder subjected to ischemic/reperfusion (I/R) injuries received little attention. To fill this gap, the present study intended to examine testosterone and orchiectomy effects on male rabbits subjected to I/R damages. Aim: To elucidate the effects of testosterone and orchiectomy on contractile response, bladder morphology, interstitial fibrosis, and oxidative stress in male rabbit bladder subjected to I/R surgery. Methods: Male New Zealand rabbits were distributed into five groups as follows: Group 1 received sham surgical procedure. In group 2, I/R surgery was performed. In group 3, testosterone (100μg/kg/day) was intramuscularly injected prior to I/R surgery. In group 4, orchiectomy was performed prior to I/R surgery. In group 5, orchiectomy was performed with subsequent testosterone administration, followed by I/R surgery. All the rabbits were euthanized 7 days after I/R. Comparative studies were analyzed to elucidate the effects of testosterone and orchiectomy on bladder dysfunction subjected to I/R injuries. Main Outcome Measures: Bladder contractile function was evaluated. Masson's trichrome staining and immunohistochemical studies were performed to evaluate bladder morphology and intramural nerve terminals. Western blotting was examined to investigate the expressions of fibrosis and oxidative stress markers. Results: I/R surgery significantly decreased bladder contractility in response to various stimulations with and without testosterone treatment. I/R damages decreased bladder nerve density with and without testosterone. The expressions of fibrosis and oxidative stress-related proteins were increased by I/R injuries with or without testosterone treatment. Testosterone depletion significantly decreased the expressions of transforming growth factor-β and fibronectin expressions after I/R injury. Supraphysiological testosterone treatment after orchiectomy greatly increased the expressions of these fibrosis proteins; however, orchiectomy alone ameliorated I/R injuries. Conclusions: Testosterone treatment or orchiectomy affected I/R-induced bladder damages in male rabbits. Orchiectomy decreased the level of fibrosis and oxidative stress markers and increased neurofilament densities. Supraphysiological exogenous testosterone administration after orchiectomy further exacerbated such detrimental effects of I/R.
机译：简介：睾丸激素和睾丸切除术在遭受缺血/再灌注（I / R）损伤的男性膀胱中的作用很少受到关注。为了填补这一空白，本研究旨在检查睾丸激素和睾丸切除术对遭受I / R损伤的雄性兔子的影响。目的：阐明睾丸激素和睾丸切除术对接受I / R手术的雄性兔膀胱收缩反应，膀胱形态，间质纤维化和氧化应激的影响。方法：将雄性新西兰兔分为五组：第1组接受假手术。在第2组中，进行了I / R手术。在第3组中，在I / R手术前肌肉注射睾丸激素（100μg/ kg /天）。在第4组中，在I / R手术之前进行睾丸切除术。在第5组中，进行睾丸切除术并随后施用睾丸激素，然后进行I / R手术。 I / R后7天将所有兔子安乐死。分析了比较研究，以阐明睾丸激素和睾丸切除术对遭受I / R损伤的膀胱功能障碍的影响。主要观察指标：评估膀胱收缩功能。进行了Masson的三色染色和免疫组化研究，以评估膀胱形态和壁内神经末梢。检查蛋白质印迹以研究纤维化和氧化应激标志物的表达。结果：在有或没有睾丸激素治疗的情况下，I / R手术都会显着降低膀胱收缩力，以应对各种刺激。 I / R损伤在有或没有睾丸激素的情况下都会降低膀胱神经密度。在有或没有睾丸激素治疗的I / R损伤中，纤维化和氧化应激相关蛋白的表达增加。 I / R损伤后，睾丸激素耗竭显着降低了转化生长因子-β的表达和纤连蛋白的表达。睾丸切除术后的超生理性睾丸激素治疗大大增加了这些纤维化蛋白的表达。但是，仅睾丸切除术可以减轻I / R损伤。结论：睾丸激素治疗或睾丸切除术会影响雄性兔I / R引起的膀胱损伤。睾丸切除术降低了纤维化和氧化应激指标的水平，并增加了神经丝的密度。睾丸切除术后给予超生理外源性睾丸激素进一步加剧了I / R的这种不利影响。

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