首页> 外文期刊>The journal of sexual medicine >Proceedings of the 16th annual congress of the European society for sexual medicine and the 12th congress of the European sexology Federation, istanbul, Turkey, january 29-february 1, 2014.
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Proceedings of the 16th annual congress of the European society for sexual medicine and the 12th congress of the European sexology Federation, istanbul, Turkey, january 29-february 1, 2014.

机译:2014年1月29日至2月1日,在土耳其伊斯坦布尔举行的欧洲性医学学会第16届年度大会和欧洲性学联合会第12届大会的会议记录。

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Objective: The aim of this study is to test the effects of a local injection of human adipose tissue-derived stem cells (ADSCs) in the chronic phase of a rat model of PD. Methods: A total of 21 male rats were divided in three equal groups and underwent injection of vehicle (sham), 0.5-microg transforming growth factor (TGF)-bl in a 50-microl vehicle in either a PD or a PD plus human ADSC group in the dorsal aspect of the TA. The sham and PD groups were treated 1 month after vehicle or TGF-bl injection with intralesional treatment of vehicle, and the ADSC group received 1 million human-labeled ADSCs in the 50-microl vehicle. 10 weeks after treatment, following euthanasia, penises were harvested for histology and Western blot analysis(WB). Quantification of signals on the PVDF membrane was performed using Kodak Image Station 440 and SigmaScan Pro 5.0 software. Comparisons were performed with an ANOVA.
机译:目的:本研究的目的是测试局部注射人脂肪组织源性干细胞(ADSC)在PD大鼠模型的慢性期中的作用。方法:将21只雄性大鼠分为三等份,分别在PD或PD加人ADSC中,在50微升载体中注射媒介物(假),0.5微克转化生长因子(TGF)-bl。组在TA背面。假手术组和PD组在媒介物或TGF-b1注射后1个月接受媒介物病灶内治疗,ADSC组在50微升媒介物中接受了100万个人标记的ADSCs。治疗后10周,安乐死后,收获阴茎用于组织学和Western blot分析(WB)。使用Kodak Image Station 440和SigmaScan Pro 5.0软件对PVDF膜上的信号进行定量。用方差分析进行比较。

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