• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Korean Journal of Genetics >Nuclear localization signal in human hnRNP L
【24h】

Nuclear localization signal in human hnRNP L

机译：人类hnRNP L中的核定位信号

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The heterogeneous nuclear ribonucleoprotein (hnRNP) L is an abundant nuclear protein and is one of the major pre-mRNA binding proteins in human cells. The amino acid sequence of hnRNP L contains four loosely conserved RNP-concensus RNA-binding domains. In previous report, it was shown that the amino terminal 140 amino acids of the hnRNP L were necessary and sufficient for nuclear localization. In order to define the minimal region of NLS in hnRNP L, a series of amino terminal and carboxy terminal deletions of hnRNP L were fused to the 3' end of the myc epitope-tagged chicken muscle pyruvate kinase (PK) cDNA. The subcellular distribution of transiently expressed polypeptides was examined by immunofluorescence microscopy. Here we report that the nuclear localization signal (NLS) sequence of hnRNP L protein consists of 24-GRAPKRLKT-32 sequences and seems to be a member of classical NLS, the single basic domain. The construct with amino terminal 23 amino acids deletion is still able to confer complete nuclear localization onto PK, a cytoplasmic reporter protein. However, the shorter construct in which amino terminal 35 amino acids were deleted completely lost the capability of targeting of cytoplasmic PK to the nucleus.
机译：异质核核糖核蛋白（hnRNP）L是一种丰富的核蛋白，是人细胞中主要的前mRNA结合蛋白之一。 hnRNP L的氨基酸序列包含四个保守的RNP共有RNA结合结构域。在先前的报告中，表明hnRNP L的氨基末端140个氨基酸对于核定位是必要和充分的。为了在hnRNP L中定义NLS的最小区域，将hnRNP L的一系列氨基末端和羧基末端缺失融合到myc表位标记的鸡肌肉丙酮酸激酶（PK）cDNA的3'末端。通过免疫荧光显微镜检查瞬时表达的多肽的亚细胞分布。在这里，我们报道hnRNP L蛋白的核定位信号（NLS）序列由24-GRAPKRLKT-32序列组成，似乎是经典NLS（单个基本域）的成员。具有氨基末端23个氨基酸缺失的构建体仍然能够将完整的核定位赋予细胞质报道蛋白PK。然而，其中缺失了氨基末端35个氨基酸的较短的构建体完全丧失了将细胞质PK靶向核的能力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号