首页> 外文期刊>The Korean Journal of Genetics >EST Analysis of Gene Expression in the Germ-layer Formation Stage of the Bombyx mori
【24h】

EST Analysis of Gene Expression in the Germ-layer Formation Stage of the Bombyx mori

机译:家蚕生殖层形成阶段基因表达的EST分析

获取原文
获取原文并翻译 | 示例
           

摘要

In order to explore the gene expression underlying early embyogenesis, expressed sequence tag (EST) analysis was conducted using a cDNA library made from the 24h embryo of a silkworm, Bombyx mori. A total of 8,928 clones were screened plasmid with cDNA insert and have partially sequenced the 5' ends to generate 2,446 assembled sequences. The annotation of 2,446 high-quality ESTs by a BLAST search, revealed that 1,516 (62%) of the sequences represented known genes. Over 2,400 ESTs consisted of 1,568 single ESTs (singlets) and 878 redundant ESTs (contigs). The functional groups of those sequences with matches in the database (P
机译:为了探索早期胚发生的基因表达,使用了由家蚕(Bombyx mori)的24小时胚胎制成的cDNA文库进行了表达序列标签(EST)分析。总共筛选了8,928个具有cDNA插入片段的质粒,并对5'端进行了部分测序,以生成2,446个组装的序列。通过BLAST搜索对2,446个高质量EST的注释显示,有1,516个(62%)序列代表了已知基因。超过2400个EST由1568个单个EST(单个)和878个冗余EST(重叠群)组成。根据推定的分子功能,生物学过程和细胞成分,构建了数据库中具有匹配项的那些序列的功能组(P

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号