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首页> 外文期刊>The Korean Journal of Genetics >Gene Expression Profiles in Mouse Pachytene Spermatocytes and Round Spermatids Revealed by cDNA Microarray
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Gene Expression Profiles in Mouse Pachytene Spermatocytes and Round Spermatids Revealed by cDNA Microarray

机译：cDNA芯片揭示的小鼠粗线精原细胞和圆形精子的基因表达谱

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摘要

Spermatogenesis is a highly complex process and many genes are involved in its regulation, but relatively few genes have been identified. To gain a comprehensive view of gene expression and of the regulatory mechanism involved in germ cell development, we used a mouse 8.0K cDNA chip to compare the gene expression profiles of mouse pachytene spermatocytes and round spermatids, which were isolated from 9-week ICR mice testes by velocity sedimentation under unit gravity (VSUG). We verified differentially expressed genes by semi-quantitative and real-Time RT-PCR, and found that the expression of 32 genes was higher in pachytene spermatocytes than in round spermatids, and 25 in round spermatids than in pachytene spermatocytes. In pachytene spermatocytes,the expression of cell cycle and proliferation-related genes, CDC2a, CDK2, CDC7, CKS2 and PCNA was increased over two-fold. In round spermatids, the expression levels of ubiquitin-related genes, USP16, USP9X and Ube2n were up regulated. The expression of the RNA binding motif (RBM) protein, RBMX, was increased over two-fold in pachytene spermatocytes, and RBM4 was increased in round spermatids. The genes identified include; primarily chromosomal genes related to intracellular enzyme and nucleic acid binding proteins, and kinase and substrate genes that mediate cell cycle transition. This dynamic gene expression and localization during spermatogenesis may be critical for the meiotic cell division process and germ cell development.
机译：精子形成是一个高度复杂的过程，许多基因参与其调控，但已鉴定出的基因相对较少。为了全面了解基因表达以及涉及生殖细胞发育的调控机制，我们使用了小鼠8.0K cDNA芯片比较了从9周ICR小鼠中分离的小鼠粗精细胞和圆形精细胞的基因表达谱。睾丸通过单位重力下的速度沉降（VSUG）。我们通过半定量和实时RT-PCR验证了差异表达的基因，发现32种基因的表达在粗线精子细胞中比圆形精子细胞高，而在圆形精子细胞中25种基因比在粗线精子细胞中高。在粗线精原细胞中，细胞周期和增殖相关基因CDC2a，CDK2，CDC7，CKS2和PCNA的表达增加了两倍。在圆形精子细胞中，泛素相关基因USP16，USP9X和Ube2n的表达水平上调。 RNA结合基序（RBM）蛋白RBMX的表达在粗线精子细胞中增加了两倍以上，而在圆形精子细胞中RBM4则增加了。确定的基因包括;主要是与细胞内酶和核酸结合蛋白有关的染色体基因，以及介导细胞周期转换的激酶和底物基因。精子发生过程中这种动态基因表达和定位可能对减数分裂细胞分裂过程和生殖细胞发育至关重要。

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