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首页> 外文期刊>The Korean Journal of Parasitology >Effects of Mannose on Pathogenesis of Acanthamoeba castellanii
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Effects of Mannose on Pathogenesis of Acanthamoeba castellanii

机译：甘露糖对卡氏棘阿米巴发病机理的影响

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摘要

Acanthamoeba spp. are single-celled protozoan organisms that are widely distributed in the environment. In this study, to understand functional roles of a mannose-binding protein (MBP), Acanthamoeba castellanii was treated with methyl-alpha-D-mannopyranoside (mannose), and adhesion and cytotoxicity of the amoeba were analyzed. In addition, to understand the association of MBP for amoeba phagocytosis, phagocytosis assay was analyzed using non-pathogenic bacterium, Escherichia coli K12. Amoebae treated with mannose for 20 cycles exhibited larger vacuoles occupying the most area of the amoebic cytoplasm in comparison with the control group amoebae and glucose-treated amoebae. Mannose-selected amoebae exhibited lower levels of binding to Chinese hamster ovary (CHO) cells. Exogenous mannose inhibited >50% inhibition of amoebae (control group) binding to CHO cells. Moreover, exogenous mannose inhibited amoebae (i.e., man-treated) binding to CHO cells by <15%. Mannose-selected amoebae exhibited significantly decreased cytotoxicity to CHO cells compared with the control group amoebae, 25.1% vs 92.1%. In phagocytic assay, mannose-selected amoebae exhibited significant decreases in bacterial uptake in comparison with the control group, 0.019% vs 0.03% (P<0.05). Taken together, it is suggested that mannose-selected A. castellanii trophozoites should be severely damaged and do not well interact with a target cell via a lectin of MBP.
机译：棘阿米巴属是在环境中广泛分布的单细胞原生动物生物。在这项研究中，为了了解甘露糖结合蛋白（MBP）的功能作用，用甲基α-D-甘露吡喃糖苷（甘露糖）处理了棘阿米巴棘阿米巴，并分析了变形虫的粘附和细胞毒性。另外，为了了解MBP与变形虫吞噬作用的关系，使用非病原性细菌大肠杆菌K12对吞噬作用分析进行了分析。与对照组变形虫和葡萄糖处理的变形虫相比，用甘露糖处理20个周期的变形虫表现出较大的液泡，占据了最大的阿米巴细胞质区域。甘露糖选择的变形虫显示出较低水平的与中国仓鼠卵巢（CHO）细胞的结合。外源性甘露糖抑制变形虫（对照组）与CHO细胞结合的抑制率> 50％。此外，外源甘露糖抑制变形虫（即，人处理的）与CHO细胞的结合＜15％。与对照组变形虫相比，甘露糖选择的变形虫对CHO细胞的细胞毒性显着降低，分别为25.1％和92.1％。在吞噬试验中，与对照组相比，甘露糖选择的变形虫显示出细菌摄取的显着降低，分别为0.019％和0.03％（P <0.05）。两者合计，建议应严格破坏甘露糖选择的卡氏曲霉滋养体，并且不能通过MBP的凝集素与靶细胞良好相互作用。

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