Microsatellite DNA Somaclonal Variation of Regenerated Plants via Cotyledon Culture of Hot Pepper (Capsicum annuum L.)

Tae-Hun Ryu; Seung-In Yi; Yong-Sham Kwon; Byung-Dong Kim

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Microsatellite DNA Somaclonal Variation of Regenerated Plants via Cotyledon Culture of Hot Pepper (Capsicum annuum L.)

机译：通过辣椒子叶培养再生植株的微卫星DNA体细胞克隆变异。

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In this study, microsatellite DNA markers were utilized to characterize somaclonal variation in regenerated plants from cultured cotyledons of Capsicum annuum L. cv Subicho. Twenty five microsatellite primer pairs were utilized in the screening of 41regenerated plants derived from the cotyledons. Microsatellite DNA variation was detected among the regenerated plants at two SSR loci. The primer pairs for the SSR loci HpmsF15 and HpmsF037 revealed somaclonal variation in 5 out of the 41 plantlets (12%). However, 23 microsatellite markers evidenced no nuclear DNA polymorphisms. Nevertheless, the microsatellite marker technique can be successfully employed to assess genetic variations in somaclonal variants of the hot pepper.

机译：在这项研究中，微卫星DNA标记被用来表征辣椒（Capsicum annuum L. cv Subicho）的子叶再生植物中体细胞克隆的变异。二十五对微卫星引物被用于筛选41个衍生自子叶的再生植物。在两个SSR位点的再生植物中检测到微卫星DNA变异。 SSR基因座HpmsF15和HpmsF037的引物对显示41株幼苗中有5株的体细胞克隆变异（12％）。但是，有23个微卫星标记没有核DNA多态性。然而，微卫星标记技术可以成功地用于评估辣椒的体细胞克隆变异中的遗传变异。

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《The Korean Journal of Genetics》 |2007年第4期|共6页
作者
Tae-Hun Ryu; Seung-In Yi; Yong-Sham Kwon; Byung-Dong Kim;
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正文语种 eng
中图分类遗传学;
关键词
cotyledon culture; microsatellite marker; pepper; somaclonal variation;

机译：子叶培养;微卫星标记;辣椒;体克隆变异;

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专利

1. 辣椒胞质雄性不育系(CMS)子叶培养植株再生 [J] . 邓明华, 文锦芬, 邹学校农业科学与技术（英文版） . 2009,第001期
2. Microsatellite DNA Somaclonal Variation of Regenerated Plants via Cotyledon Culture of Hot Pepper (Capsicum annuum L.) [J] . Tae-Hun Ryu, Seung-In Yi, Yong-Sham Kwon, The Korean Journal of Genetics . 2007,第4期

机译：通过辣椒子叶培养再生植株的微卫星DNA体细胞克隆变异。
3. High-quality embryo production and plant regeneration using a two-step culture system in isolated microspore cultures of hot pepper (Capsicum annuum L.) [J] . Moonza Kim, Eun-Joon Park, Dongju An, Plant Cell, Tissue and Organ Culture (PCTOC) . 2013,第2期

机译：在辣椒（Capsicum annuum L.）的分离小孢子培养中，使用两步培养系统进行高质量的胚胎生产和植物再生
4. High-quality embryo production and plant regeneration using a two-step culture system in isolated microspore cultures of hot pepper (Capsicum annuum L.) [J] . Kim Moonza, Park Eun-Joon, An Dongju, Plant Cell, Tissue and Organ Culture: An International Journal on in Vitro Culture of Higher Plants . 2013,第2期

机译：在辣椒（Capsicum annuum L.）的分离小孢子培养中，使用两步培养系统进行高质量的胚胎生产和植物再生
5. Differential gene expression in cell cultures and plants of chili pepper (Capsicum annuum I.) under water stress [C] . N. OCHOA-AlEJO, Y.M. CAMACHO-VlLLASANA Workshop of the International Maize and Wheat Improvement Center . 2004

机译：水分胁迫下辣椒（Capsicum Anuum I.）细胞培养物和植物中的差异基因表达
6. In-vitro propagation of Capsicum annuum cv. early prolific hybrid pepper and evaluation of regenerated plants. [D] . Cooke, Margaret Anne. 1988

机译：辣椒的体外繁殖。早期多产杂种辣椒和再生植物的评价。
7. An improved plant regeneration and Agrobacterium - mediated transformation of red pepper (Capsicum annuum L.) [O] . R. Vinoth Kumar, V. K. Sharma, B. Chattopadhyay, 2012

机译：改良的植物再生和农杆菌介导的红辣椒（辣椒）的转化
8. Plant Regeneration of Hot Pepper (Capsicum annuum L.) and Expression of Mouse Adenosine Deaminase (ADA) Gene via Agrobacterium-mediated Transformation [O] . Hak-Tae Lim, Kei-youn Lee, Yeoung-Sook Yoo, 1996

机译：盐辣椒（辣椒Annum L.）的植物再生和小鼠腺苷脱氨酶（ADA）基因通过农杆菌介导的转化的表达

Microsatellite DNA Somaclonal Variation of Regenerated Plants via Cotyledon Culture of Hot Pepper (Capsicum annuum L.)

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