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首页> 外文期刊>The Korean Journal of Parasitology >Detection of Plasmodium vivax by Nested PCR and Real-Time PCR
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Detection of Plasmodium vivax by Nested PCR and Real-Time PCR

机译：巢式PCR和实时荧光定量PCR检测间日疟原虫

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摘要

Malaria is endemic in the Cukurova region while it is sporadic in other regions of Turkey. Therefore, the laboratory and clinical diagnosis of malaria is important for the treatment of malaria. In this study, 92 blood samples that were taken from the suspected malaria patients for routine diagnosis in a period of 10 years between 1999 and 2009 were analyzed. All of these blood samples were examined by microscopic examinations using Giemsa-stained thick blood films, nested PCR, and real-time PCR. The sensitivity-specificity and positive-negative predictive values for these diagnostic tests were then calculated. It was found that the positive predictive values of microscopic examination of thick blood films, nested PCR, and real-time PCR were 47.8%, 56.5%, and 60.9% for malaria, respectively. The real-time PCR was found to have a specificity of 75% and sensitivity of 100%, while specificity and sensitivity of nested PCR was found 81.2% and 97.7% according to the microscopic examination of thick blood films, respectively.
机译：疟疾在库库洛娃地区很流行，而在土耳其其他地区则是零星的。因此，疟疾的实验室和临床诊断对于治疗疟疾很重要。在这项研究中，分析了从1999年至2009年的10年中从可疑疟疾患者中进行常规诊断的92份血液样本。使用吉姆萨染色的厚血膜，巢式PCR和实时PCR通过显微镜检查检查所有这些血液样本。然后计算出这些诊断测试的敏感性特异性和阳性阴性预测值。结果发现，对于疟疾，镜检厚血膜，巢式PCR和实时PCR的阳性预测值分别为47.8％，56.5％和60.9％。实时PCR的特异度为75％，敏感性为100％，而巢式PCR的特异度和敏感性根据厚血膜的显微镜检查分别为81.2％和97.7％。

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