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首页> 外文期刊>The Journal of Thoracic and Cardiovascular Surgery >Effects of cryopreservation on contractile properties of porcine isolated aortic valve leaflets and aortic wall.
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Effects of cryopreservation on contractile properties of porcine isolated aortic valve leaflets and aortic wall.

机译：冷冻保存对猪离体主动脉瓣小叶和主动脉壁收缩特性的影响。

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摘要

Human semilunar donor heart valves can be stored in banks, awaiting transplantation. To evaluate the result of the preservation protocols, a quantitative description of the tissue is necessary. In this study we investigated in a quantitative way the contractile properties of fresh and cryopreserved porcine isolated aortic heart valve leaflets in response to a number of endogenous vasoactive compounds. The responses of strips of the aortic wall were included for comparison. Contraction was measured isometrically in response to potassium (K+; 100 mmol/L), 5-hydroxytryptamine (1 nmol/L to 100 micromol/L), noradrenaline (1 nmol/L to 100 micromol/L), endothelin-1 (0.01 nmol/L to 0.3 micromol/L), and prostaglandin F(2alpha) (0.1 nmol/L to 10 micromol/L). The pharmacologic parameters E(MAX) (the maximal response expressed as a percentage of contraction to a 100 mmol/L dose of K+) and EC50 (the concentration that produces 50% of the maximal effect) were calculated for every compound (n = 6 to 7 each). We observed that all specimens contracted in response to potassium. Its magnitude in fresh leaflets equaled 1.6 +/- 0.14 mN compared with 26.6 +/- 2.6 mN in fresh aortic wall. Noradrenaline, endothelin-1, and prostaglandin F(2alpha) all caused contraction in valvular leaflets and aortic wall, whereas 5-hydroxytryptamine caused contraction in the valvular leaflets but relaxation in aortic wall. After cryopreservation, the response to K+ amounted to 24% of the response of the fresh specimens in valvular leaflets (n = 25) and 14% in aortic wall (n = 26). The values of E(MAX) and EC50 of the responses to noradrenaline, endothelin-1, and prostaglandin F(2alpha) remained unchanged. Although the physiologic relevance of contraction of valvular leaflets needs further study, its measurement may provide an additional model to verify the consequences of alternative methods of preservation.
机译：人类半月供体心脏瓣膜可以储存在库中，等待移植。为了评估保存方案的结果，必须对组织进行定量描述。在这项研究中，我们以定量的方式研究了新鲜和冷冻保存的猪分离的主动脉心脏瓣膜小叶对许多内源性血管活性化合物的收缩特性。比较主动脉壁条的反应。对钾（K +; 100 mmol / L），5-羟基色胺（1 nmol / L至100 micromol / L），去甲肾上腺素（1 nmol / L至100 micromol / L），内皮素-1（0.01）进行等距测量nmol / L至0.3 micromol / L）和前列腺素F（2alpha）（0.1 nmol / L至10 micromol / L）。计算每种化合物的药理参数E（MAX）（最大反应，以对100 mmol / L剂量K +的收缩百分比表示）和EC50（产生最大作用的浓度50％）（n = 6）至7个）。我们观察到所有标本均响应钾离子收缩。新鲜小叶的大小为1.6 +/- 0.14 mN，而新鲜主动脉壁的大小为26.6 +/- 2.6 mN。去甲肾上腺素，内皮素-1和前列腺素F（2alpha）都引起瓣膜小叶和主动脉壁的收缩，而5-羟色胺引起了瓣膜小叶的收缩但主动脉壁的松弛。冷冻保存后，对K +的响应相当于新鲜标本在瓣膜小叶中的响应的24％（​​n = 25）和主动脉壁中14％的响应（n = 26）。对去甲肾上腺素，内皮素-1和前列腺素F（2alpha）的响应的E（MAX）和EC50值保持不变。尽管瓣膜小叶收缩的生理相关性需要进一步研究，但其测量可能会提供一个附加模型来验证其他保存方法的后果。

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