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首页> 外文期刊>The Journal of toxicological sciences >Morphological characterization of the ovary undernormal cycling in rats and its viewpoints ofovarian toxicity detection
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Morphological characterization of the ovary undernormal cycling in rats and its viewpoints ofovarian toxicity detection

机译：大鼠卵巢功能异常循环的形态学特征及卵巢毒性检测的观点

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Identification of ovarian toxicity is very important for safety assessment of drugs and other environmental chemicals. The detection of interference with ovarian function is very hard without a thorough understanding of the normal ovarian morphology based on reproductive physiology. The focus of the present study was therefore a practical analysis in each stage of the estrous cycles using ovaries obtained from 143 rats demonstrating normal cycling. Transversely dissected maximum areas in the ovaries were examined microscopically for the two major features, follicles and corpora lutea (CL). Classification of growing follicles was in reference to Pedersen and Peters (1968), and functionally divided into follicular stimulating hormone (FSH)-independent and dependent categories. The former, small and medium-sized follicles, respectively primordial/primary and preantral follicles, could be readily detected by immunohistochemical staining for proliferating cell nuclear antigen (PCNA). The large antral and Graa-fian follicles and large sized atretic follicles showed sequential changes depending on the estrous cycle stage. CL could be divided into currently and previously formed examples. Currently formed CL underwent remarkable changes in their appearance with the cycle, reflecting ovulation and progesterone production. Thus morphological analysis that is synchronized the large antral follicle changes with recently formed CL ones allows the ovary to be classified into the each estrous cycle stage. Morphological deviation from any synchronized combination provides a first pointer of ovarian toxicity. PCNA immunohistochemical staining is also useful to detect small follicles.
机译：卵巢毒性的鉴定对于药物和其他环境化学品的安全性评估非常重要。如果没有基于生殖生理学对正常卵巢形态的透彻了解，很难检测到卵巢功能受到干扰。因此，本研究的重点是在动情周期的每个阶段中使用从143只大鼠中获得的卵巢进行正常周期的卵巢分析。显微剖视卵巢的最大横截面积，以检查卵泡和黄体（CL）这两个主要特征。生长卵泡的分类参考Pedersen和Peters（1968），在功能上分为独立于卵泡刺激激素（FSH）和依赖的两类。通过增殖细胞核抗原（PCNA）的免疫组织化学染色可以很容易地检测到前，中，小型卵泡，分别是原始/初级和窦前卵泡。较大的肛门和Graa-fian卵泡以及较大的Atretic卵泡根据发情周期阶段显示顺序变化。 CL可以分为当前和先前形成的示例。目前形成的CL的外观随周期发生显着变化，反映了排卵和孕酮的产生。因此，形态学分析与最近形成的CL囊泡同步使大的肛门卵泡变化同步，从而使卵巢可以分为每个发情周期阶段。与任何同步组合的形态学差异为卵巢毒性提供了首要指标。 PCNA免疫组化染色也可用于检测小卵泡。

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