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首页> 外文期刊>The Journal of toxicological sciences >Cellular effects of industrial metal nanoparticles and hydrophilic carbon black dispersion
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Cellular effects of industrial metal nanoparticles and hydrophilic carbon black dispersion

机译：工业金属纳米颗粒和亲水性炭黑分散体的细胞效应

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摘要

The effects of five types of metal nanoparticles, gold (Au), silver (Ag), platinum (Pt), Au-polyvinylpyrrolidone (PVP) colloid, and Pt-PVP colloid, and two types of hydrophilic carbon black on cell behavior were examined. Stable nanoparticle dispersions were prepared and applied to the culture medium of human keratinocyte (HaCaT) and human lung carcinoma (A549) cells for 6 and 24 hr. Then, the mitochondrial activity (MTT assay) and the induction of cellular oxidative stress were examined. The exposure to Au and Ag decreased mitochondrial activity. The exposure to Pt nanoparticles induced an increase in the intracellular reactive oxygen species (ROS) level. In contrast, Au-PVP, Pt-PVP, and hydrophilic carbon black did not exhibit any effects. The observed increase in the ROS level induced by the Pt nanoparticles in this study contradicted our previous findings, in which Pt did not produce chemically reactive molecules. Some nanoparticle dispersions included chemicals as the dispersant, which is used in industrial applications. In some cases, the dispersing agent may have caused some cellular effects. Adsorption of agents on the surface of the nanoparticles may be an important factor here. Hence, the cellular effects of industrial nanoparticles should be evaluated carefully.
机译：研究了五种类型的金属纳米颗粒，金（Au），银（Ag），铂（Pt），金聚乙烯吡咯烷酮（PVP）胶体和Pt-PVP胶体以及两种类型的亲水性炭黑对细胞行为的影响。制备稳定的纳米颗粒分散体，并将其应用于人角质形成细胞（HaCaT）和人肺癌（A549）细胞的培养基中6和24小时。然后，检查线粒体活性（MTT测定）和细胞氧化应激的诱导。暴露于金和银会降低线粒体活性。暴露于Pt纳米颗粒会引起细胞内活性氧（ROS）水平的增加。相反，Au-PVP，Pt-PVP和亲水性炭黑未显示任何作用。在这项研究中观察到的由Pt纳米颗粒诱导的ROS水平升高与我们先前的发现相矛盾，在先前的发现中，Pt不产生化学反应性分子。一些纳米粒子分散体包括化学物质作为分散剂，用于工业应用。在某些情况下，分散剂可能已引起某些细胞效应。在此，试剂在纳米颗粒表面上的吸附可能是重要的因素。因此，应仔细评估工业纳米颗粒的细胞效应。

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