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首页> 外文期刊>The Journal of toxicological sciences >Development of LLNA:DAE: A new local lymph node assay that includes the elicitation phase, discriminates borderline-positive chemicals, and is useful for cross-sensitization testing
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Development of LLNA:DAE: A new local lymph node assay that includes the elicitation phase, discriminates borderline-positive chemicals, and is useful for cross-sensitization testing

机译：LLNA：DAE的开发：一种新的局部淋巴结测定法，包括诱导阶段，可区分临界阳性化学物质，可用于交叉敏化测试

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We developed a new local lymph node assay (LLNA) that includes the elicitation phase termed LLNA:DAE for discrimination of borderline-positive chemicals as classified by the LLNA modified by Daicel based on ATP content (LLNA:DA) and for cross-sensitization testing. Although the LLNA:DA method could help identify skin sensitizers, some skin irritants classified as non-sensitizers by the LLNA were classified as borderline positive. In addition, the evaluation for the cross-sensitization potential between chemicals was impossible. In the LLNA:DAE procedure, test group of mice received four applications of chemicals on the dorsum of the right ear for induction and one application on the dorsum of the left ear for elicitation. Control group of mice received one chemical application on the dorsum of the left ear. We evaluated the sensitizing potential by comparing the weights of the lymph nodes from the left ears between the two groups. The results of using the LLNA:DAE method to examine 24 chemicals, which contained borderline-positive chemicals, were consistent with those from the LLNA method, except for nickel chloride (NiCl2). Two chemical pairs, 2,4-dinitrochlorobenzene (DNCB) with 2,4-dinitrofluorobenzene (DNFB) and hydroquinone (HQ) with p-benzoquinone (p-BQ), showed clear cross-sensitization with each other, while another chemical pair, DNFB with hexylcinnamic aldehyde (HCA) did not. Taken together, our results suggest that the LLNA:DAE method is useful for discriminating borderline-positive chemicals and for determining chemical cross-sensitization.
机译：我们开发了一种新的局部淋巴结测定法（LLNA），其中包括称为LLNA：DAE的诱导阶段，用于区分边缘阳性化学药品（由Daicel修改后的LLNA根据ATP含量（LLNA：DA）对其进行分类并进行交叉敏化测试。尽管LLNA：DA方法可以帮助识别皮肤敏化剂，但LLNA归类为非敏化剂的某些皮肤刺激物被归为阳性。另外，不可能评估化学品之间的交叉致敏性。在LLNA：DAE程序中，测试组的小鼠在右耳的背部接受了四种化学药品的诱导，在左耳的背部接受了一种化学试剂的诱导。对照组小鼠在左耳背上接受了一种化学疗法。我们通过比较两组左耳淋巴结的重量来评估其致敏潜力。使用LLNA：DAE方法检查24种包含临界阳性化学物质的结果与LLNA方法的结果一致，除了氯化镍（NiCl2）。两个化学对，分别是带有2,4-二硝基氟苯（DNFB）的2,4-二硝基氯苯（DNCB）和带有对苯醌（p-BQ）的氢醌（HQ），彼此之间具有明显的交叉敏化作用，而另一个化学对DNFB与己基肉桂醛（HCA）没有。综上所述，我们的结果表明LLNA：DAE方法可用于区分临界阳性化学物和确定化学交叉敏化作用。

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