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首页> 外文期刊>The Journal of toxicological sciences >Arsine toxicity is induced by inhalation but not by percutaneous exposure in hairless mice
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Arsine toxicity is induced by inhalation but not by percutaneous exposure in hairless mice

机译：在无毛小鼠中，吸入而不是经皮接触可引起砷化氢中毒

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Arsine (AsH3) is used in many industries, but there is insufficient knowledge about the potential for percutaneous absorption. In order to examine possible percutaneous absorption of arsine, we conducted inhalation studies. Arsine was generated by reducing arsenic trioxide with NaBH4. Male 5-week-old Hos:HR-1 hairless mice were subjected to a single percutaneous exposure or wholebody inhalation exposure of ca. 300 ppm arsine for 5 min. The examination was performed 0-6 hr after the exposure. Total arsenic in whole blood and hematocrit (Ht) values were measured. Generation of an arsenic-hemoglobin (As-Hb) adduct in the blood was detected using high-performance liquid chromatography with an inductively coupled plasma mass spectrometer (HPLC-ICP-MS). Ht values in the inhalation group significantly decreased after 3 hr, but those in the percutaneous exposure group did not. Total arsenic in the inhalation group was 9.0-14.2 mg/l, which was significantly higher than that in the percutaneous group. The As-Hb adduct was detected only in mice in the inhalation group. Histopathological changes were noted only in the inhalation group, with marked deposition of eosinophilic globules in the proximal convoluted tubules of the kidneys, the Kupffer cells of the liver, and the red pulp in the spleen, but not in the lungs. Immunohistochemically, these eosinophilic globules were stained positively by hemoglobin (Hb) antibody. In the present study, arsine-induced hemolysis and deposition of Hb occurred in the kidney via the inhalation route but not via percutaneous exposure. The presence of As-Hb adduct may be a useful indicator for confirming arsine poisoning.
机译：砷化氢（AsH3）已用于许多行业，但对经皮吸收潜力的了解不足。为了检查可能的经皮吸收的rs，我们进行了吸入研究。通过用NaBH4还原三氧化二砷产生generated。对5周大的Hos：HR-1雄性雄性小鼠进行约一次单次经皮暴露或全身吸入暴露。 300 ppm砷化5分钟。暴露后0-6小时进行检查。测量全血中的总砷和血细胞比容（Ht）值。使用高效液相色谱仪和电感耦合等离子体质谱仪（HPLC-ICP-MS）检测血液中砷-血红蛋白（As-Hb）加合物的生成。吸入组的Ht值在3小时后显着下降，而经皮暴露组的Ht值没有下降。吸入组总砷为9.0-14.2 mg / l，明显高于经皮组。仅在吸入组的小鼠中检测到As-Hb加合物。仅在吸入组中注意到组织病理学变化，嗜酸性小球明显沉积在肾脏的近曲形小管，肝脏的库普弗细胞和脾脏中的红色浆液中，而在肺部则没有。在免疫组织化学上，这些嗜酸性小球被血红蛋白（Hb）抗体阳性染色。在本研究中，a的诱导溶血和血红蛋白沉积是通过吸入途径而不是经皮暴露在肾脏中发生的。 As-Hb加合物的存在可能是确认砷中毒的有用指标。

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