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首页> 外文期刊>The Journal of toxicological sciences >Flow cytometric analysis of erythropoietic abnormality: changes in the cell maturity index of reticulocytes and retic distribution index are useful as indicators of erythropoietic toxicity in non-clinical studies.
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Flow cytometric analysis of erythropoietic abnormality: changes in the cell maturity index of reticulocytes and retic distribution index are useful as indicators of erythropoietic toxicity in non-clinical studies.

机译：流式细胞仪分析促红细胞生成异常：网状细胞的细胞成熟指数和网状分布指数的变化在非临床研究中可用作促红细胞生成毒性的指标。

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摘要

We performed a flow cytometric (FCM) analysis of the maturity of reticulocytes using peripheral blood obtained from rats administered 5-fluorouracil (5-FU) at 10, 50 and 100 mg/kg or acethylphenyl hydrazine (APHZ) at 1 and 3 mg/kg to clarify whether the FCM method is useful for assessing toxicity. In the 5-FU-administered rats, a decrease and recovery of the immature reticulocyte fraction (Cell Maturity Index, CMI; Retic Distribution Index, RDI) was observed more rapidly (several days prior to changes in the reticulocyte ratio), and sensitively regarding dose-dependency (clear changes were observed at 10 mg/kg, whereas the reticulocyte ratio was only slightly affected). In addition, there was good agreement between the microscopic results obtained by counting Heilmyer's reticulocyte maturation groups, especially for type I and II, and CMI/RDI assessed by the FCM method after the administration of 50 and 100 mg/kg of 5-FU, the dose at which clear changes were obtained with the microscopic method. In theAPHZ-administered rats, a dose-dependent increase in CMI/RDI coinciding with the enhancement of reticulocyte production was observed. The results suggested that the automated FCM method could be a useful and valuable tool to assess and predict impairments of erythropoiesis, especially for CMI and RDI, and could help in the diagnosis of hematological disorders in experimental animals.
机译：我们使用从以10、50和100 mg / kg的5-氟尿嘧啶（5-FU）或以1和3 mg /公斤以阐明FCM方法是否可用于评估毒性。在服用5-FU的大鼠中，未成熟的网织红细胞分数（细胞成熟度指数，CMI;网状分布指数，RDI）的降低和恢复被观察到更快（网织红细胞比例变化前几天），并且剂量依赖性（在10 mg / kg时观察到明显的变化，而网织红细胞比例仅受到轻微影响）。此外，在计数50和100 mg / kg的5-FU后，通过计数Heilmyer网织红细胞成熟组（尤其是I型和II型）和通过FCM方法评估的CMI / RDI得出的微观结果之间存在良好的一致性。用显微镜方法获得明显变化的剂量。在给予APHZ的大鼠中，观察到CMI / RDI的剂量依赖性增加与网织红细胞产生的增加一致。结果表明，自动化FCM方法可能是评估和预测红细胞生成障碍（尤其是CMI和RDI）的有用和有价值的工具，并有助于诊断实验动物的血液系统疾病。

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