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首页> 外文期刊>The Journal of toxicological sciences >Ubiquitin-conjugating enzyme Cdc34 mediates methylmercury resistance in Saccharomyces cerevisiae by increasing Whi2 degradation.
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Ubiquitin-conjugating enzyme Cdc34 mediates methylmercury resistance in Saccharomyces cerevisiae by increasing Whi2 degradation.

机译：泛素结合酶Cdc34通过增加Whi2降解介导酿酒酵母中的甲基汞抗性。

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摘要

Overexpression of Cdc34, a ubiquitin-conjugating enzyme, confers methylmercury resistance on yeast cells. This suggests that degradation, by the ubiquitin-proteasome (UP) system, of proteins that enhance methylmercury toxicity might be a factor in the development of methylmercury resistance. The present study shows that yeast cells overexpressing Whi2, a protein that is ubiquitinated in cells, are highly susceptible to methylmercury, suggesting that Whi2 may enhance methylmercury toxicity. Although both Whi2 deficiency and Cdc34 overexpression individually confer methylmercury resistance on yeast cells, Whi2-deficient cells overexpressing Cdc34 showed no additive resistance to methylmercury, compared with Whi2-expressing cells that overexpress Cdc34. The intracellular level of Whi2 was significantly reduced by Cdc34 overexpression; however, this reduction was almost completely attenuated when proteasomal degradation was inhibited. These results suggest that overexpression of Cdc34 confers methylmercury resistance on yeast cells through the UP system by promoting degradation of Whi2, a methylmercury toxicity-enhancing protein.
机译：泛素结合酶Cdc34的过表达赋予酵母细胞甲基汞抗性。这表明通过泛素-蛋白酶体（UP）系统降解的增强甲基汞毒性的蛋白质可能是产生甲基汞抗性的一个因素。本研究表明，过量表达Whi2（一种在细胞中泛素化的蛋白质）的酵母细胞对甲基汞高度敏感，这表明Whi2可能会增强甲基汞的毒性。尽管Whi2缺乏和Cdc34过表达都各自赋予了酵母细胞对甲基汞的抗性，但与Whi2过度表达Cdc34的细胞相比，过表达Cdc34的Whi2缺乏细胞对甲基汞没有附加的抗性。 Cdc34过表达显着降低了Whi2的细胞内水平。然而，当抑制蛋白酶体降解时，这种减少几乎完全减弱了。这些结果表明，Cdc34的过表达通过促进Whi2（甲基汞毒性增强蛋白）的降解，通过UP系统赋予酵母细胞甲基汞抗性。

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