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首页> 外文期刊>The New Phytologist >A nodule-specific plant cysteine proteinase, AsNODF32, is involved in nodule senescence and nitrogen fixation activity of the green manure legume Astragalus sinicus.
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A nodule-specific plant cysteine proteinase, AsNODF32, is involved in nodule senescence and nitrogen fixation activity of the green manure legume Astragalus sinicus.

机译：结节特异性植物半胱氨酸蛋白酶AsNODF32参与绿肥豆科植物黄芪的结节衰老和固氮活性。

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摘要

Asnodf32, encoding a nodule-specific cysteine proteinase in Astragalus sinicus, is probably involved in nodule senescence. To obtain direct evidence of its role in nodule senescence, Agrobacterium rhizogenes-mediated RNA interference was applied to A. sinicus hairy roots. Real-time qRT-PCR was used to estimate the efficiency of suppression. The senescent phenotype of transgenic nodules was examined with paraffin-embedded slides, TUNEL (TdT-mediated dUTP nick-end labeling) assay, and transmission electron microscopy, and the bacteroid nitrogen fixation activity was also measured. It was found that silencing of Asnodf32 delayed root nodule and bacteroid senescence. The period of bacteroid active nitrogen fixation was significantly extended. Interestingly, nodules enlarged in length were also observed on Asnodf32-silenced hairy roots. The results reported here indicate that Asnodf32 plays an important role in the regulation of root nodule senescence.
机译：Asnodf32编码黄芪中的一个结节特异性半胱氨酸蛋白酶，可能参与了结节衰老。为了获得其在结节衰老中作用的直接证据，将发根农杆菌介导的RNA干扰应用于中华按蚊毛状根。实时qRT-PCR用于估计抑制效率。用石蜡包埋的玻片，TUNEL（TdT介导的dUTP缺口末端标记）测定和透射电子显微镜检查了转基因结节的衰老表型，并测定了类固氮活性。已经发现，沉默Asnodf32延迟了根瘤和类细菌衰老。细菌活性氮的固定期显着延长。有趣的是，在沉默了Asnodf32的毛状根上也观察到了结节，其长度增大了。此处报道的结果表明，Asnodf32在调节根瘤衰老中起重要作用。

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