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首页> 外文期刊>The New Phytologist >Five phylogenetically close rice SWEET genes confer TAL effector-mediated susceptibility to Xanthomonas oryzae pv. oryzae
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Five phylogenetically close rice SWEET genes confer TAL effector-mediated susceptibility to Xanthomonas oryzae pv. oryzae

机译：五个系统发育上接近的水稻SWEET基因赋予TAL效应子介导的米黄单胞菌（Panthomonas oryzae pv）易感性。水稻

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摘要

Bacterial plant-pathogenic Xanthomonas strains translocate transcription activator-like (TAL) effectors into plant cells to function as specific transcription factors. Only a few plant target genes of TAL effectors have been identified, so far. Threeplant SWEET genes encoding putative sugar transporters are known to be induced by TAL effectors from rice-pathogenic Xanthomonas oryzae pv. oryzae (Xoo). We predict and validate that expression of OsSWEET14 is induced by a novel TAL effector, Tal5, froman African Xoo strain. Artificial TAL effectors (ArtTALs) were constructed to individually target 20 SWEET orthologs in rice. They were used as designer virulence factors to study which rice SWEET genes can support Xoo virulence. The Tal5 target box differs from those of the already known TAL effectors TalC, AvrXa7 and PthXo3, which also induce expression of OsSWEET14, suggesting evolutionary convergence on key targets. ArtTALs efficiently complemented an Xoo talC mutant, demonstrating that specific induction of OsSWEET14 is the key target of TalC. ArtTALs that specifically target individual members of the rice SWEET family revealed three known and two novel SWEET genes to support bacterial virulence. Our results demonstrate that five phylogeneticallyclose SWEET proteins, which presumably act as sucrose transporters, can support Xoo virulence.
机译：细菌致病性黄单胞菌菌株将转录激活因子样（TAL）效应子易位到植物细胞中，以充当特定的转录因子。到目前为止，仅鉴定了几种TAL效应子的植物靶基因。已知编码致病性糖转运蛋白的三株SWEET基因是由水稻致病性米氏黄单胞菌（Xanthomonas oryzae pv）的TAL效应子诱导的。稻（Xoo）。我们预测并验证，OsSWEET14的表达是由来自非洲Xoo菌株的新型TAL效应子Tal5诱导的。人工TAL效应子（ArtTALs）的构建可以分别针对水稻中的20个SWEET直系同源物。它们被用作设计毒力因子来研究哪些水稻SWEET基因可以支持Xoo毒力。 Tal5靶标框不同于已知的TAL效应子TalC，AvrXa7和PthXo3，后者也诱导OsSWEET14的表达，提示关键靶标的进化趋同。 ArtTALs有效补充了Xoo talC突变体，表明OsSWEET14的特异性诱导是TalC的主要靶标。专门针对水稻SWEET家族个体成员的ArtTALs显示了三个已知的和两个新的SWEET基因来支持细菌的毒性。我们的结果表明，五个系统发育上接近的SWEET蛋白（可能充当蔗糖转运蛋白）可以支持Xoo毒力。

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