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首页> 外文期刊>The New Phytologist >Mediator subunit 16 functions in the regulation of iron uptake gene expression in Arabidopsis
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Mediator subunit 16 functions in the regulation of iron uptake gene expression in Arabidopsis

机译：介体亚基16在拟南芥中调节铁吸收基因表达中的功能

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Iron is an essential nutrient for plant growth and development, and its absorption is tightly controlled. Under iron limitation, FIT dimerizes with the four Ib bHLH proteins and activates the expression of iron uptake genes. However, how the dimerized complex activates downstream genes remains unclear. Using forward genetics, a low-iron-sensitive mutant was screened. The corresponding gene (MED16) was isolated, and its biological functions in iron homeostasis were characterized using approaches such as gene expression, protein subcellular localization, protein-protein interaction and chromatin immunoprecipitation assay. Lesion of MED16 significantly reduced FRO2 and IRT1 expression in Arabidopsis roots. The MED16 mutants showed a low shoot iron concentration and severe leaf chlorosis under iron limitation, whereas it grew normally as wild-type under iron sufficiency. Furthermore, we showed that MED16 interacted with FIT and improved the binding of the FIT/Ib bHLH complex to FRO2 and IRT1 promoters under iron-deficient conditions. Additionally, we found that many iron-deficient response genes, which are regulated by FIT, were also controlled by MED16. In conclusion, MED16 is involved in the iron deficiency response, and modulates the iron uptake gene expression under iron limitation. Our results increase the understanding of the molecular regulation mechanisms underlying iron uptake and homeostasis in plants.
机译：铁是植物生长发育的必需营养素，其吸收受到严格控制。在铁的限制下，FIT与四种Ib bHLH蛋白二聚并激活铁摄取基因的表达。然而，二聚体复合物如何激活下游基因仍不清楚。使用正向遗传学，筛选了低铁敏感性突变体。分离出相应的基因（MED16），并通过基因表达，蛋白质亚细胞定位，蛋白质-蛋白质相互作用和染色质免疫沉淀等方法表征其在铁稳态中的生物学功能。 MED16病变显着降低了拟南芥根中FRO2和IRT1的表达。在铁限制下，MED16突变体显示出较低的芽生铁浓度和严重的叶片萎黄，而在铁充足的情况下，其正常生长为野生型。此外，我们显示MED16与FIT相互作用并在缺铁条件下改善FIT / Ib bHLH复合物与FRO2和IRT1启动子的结合。此外，我们发现许多受FIT调节的缺铁反应基因也受MED16的控制。总之，MED16参与铁缺乏反应，并在铁限制下调节铁摄取基因的表达。我们的结果增加了对植物体内铁吸收和体内稳态的分子调控机制的了解。

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