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首页> 外文期刊>The New Phytologist >A cotton ascorbate peroxidase is involved in hydrogen peroxide homeostasis during fibre cell development
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A cotton ascorbate peroxidase is involved in hydrogen peroxide homeostasis during fibre cell development

机译：棉花抗坏血酸过氧化物酶参与纤维细胞发育过程中的过氧化氢稳态

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摘要

Reactive oxygen species (ROS) play important roles in multiple physiological processes such as cellular signalling and stress responses, whereas, the hydrogen peroxide (H(2)O(2)) scavenging enzyme ascorbate peroxidase (APX) participates in the regulation of intracellular ROS levels. Here, a cotton (Gossypium hirsutum) cytosolic APX1 (GhAPX1) was identified to be highly accumulated during cotton fibre elongation by proteomic analysis. GhAPX1 cDNA contained an open reading frame of 753-bp encoding a protein of 250 amino acid residues. When GhAPX1 was expressed in Escherichia coli, the purified GhAPX1 was a dimer consisting of two identical subunits with a molecular mass of 28 kDa. GhAPX1 showed the highest substrate specificity for ascorbate. Quantitative real-time polymerase chain reaction (PCR) analyses showed that GhAPX1 was highly expressed in wild-type 5-d postanthesis fibres with much lower transcript levels in the fuzzless-lintless mutant ovules. Treating in vitro cultured wild-type cotton ovules with exogenous H(2)O(2) or ethylene induced the expression of GhAPX1 and hence increased total APX activity proportionally, followed by extended fibre cell elongation. These data suggest that GhAPX1 expression is upregulated in response to an increase in cellular H(2)O(2) and ethylene. GhAPX1 encodes a functional enzyme that is involved in hydrogen peroxide homeostasis during cotton fibre development.
机译：活性氧（ROS）在多种生理过程中发挥重要作用，例如细胞信号传导和应激反应，而过氧化氢（H（2）O（2））清除酶抗坏血酸过氧化物酶（APX）参与细胞内ROS的调节水平。在这里，通过蛋白质组学分析，棉花棉纤维中的APX1（GhAPX1）被高度积累。 GhAPX1 cDNA包含一个753-bp的开放阅读框，编码一个250个氨基酸残基的蛋白质。当GhAPX1在大肠杆菌中表达时，纯化的GhAPX1是由两个相同的亚基组成的二聚体，分子量为28 kDa。 GhAPX1显示出最高的抗坏血酸底物特异性。实时定量聚合酶链反应（PCR）分析表明，GhAPX1在野生型5-d花后纤维中高表达，而无绒毛的突变胚珠中的转录水平要低得多。用外源性H（2）O（2）或乙烯处理体外培养的野生型棉胚珠诱导GhAPX1的表达，因此按比例增加总APX活性，然后延长纤维细胞的伸长率。这些数据表明响应细胞H（2）O（2）和乙烯的增加，GhAPX1表达上调。 GhAPX1编码一种功能酶，该酶参与棉纤维发育过程中的过氧化氢稳态。

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