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首页> 外文期刊>The New Phytologist >Modification of nitrogen remobilization, grain fill and leaf senescence in maize (Zea mays) by transposon insertional mutagenesis in a protease gene
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Modification of nitrogen remobilization, grain fill and leaf senescence in maize (Zea mays) by transposon insertional mutagenesis in a protease gene

机译：蛋白酶基因中转座子插入诱变对玉米（Zea mays）氮转运，籽粒填充和叶片衰老的修饰

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摘要

A maize (Zea mays) senescence-associated legumain gene, See2beta, was characterized at the physiological and molecular levels to determine its role in senescence and resource allocation. A reverse-genetics screen of a maize Mutator (Mu) population identified a Mu insertion in See2beta. Maize plants homozygous for the insertion were produced. These See2 mutant and sibling wild-type plants were grown under high or low quantities of nitrogen (N). The early development of both genotypes was similar; however, tassel tip and collar emergence occurred earlier in the mutant. Senescence of the mutant leaves followed a similar pattern to that of wild-type leaves, but at later sampling points mutant plants contained more chlorophyll than wild-type plants and showed a small extension in photosynthetic activity. Total plant weight was higher in the wild-type than in the mutant, and there was a genotype x N interaction. Mutant plants under low N maintained cob weight, in contrast to wild-type plants under the same treatment. It is concluded, on the basis of transposon mutagenesis, that See2beta has an important role in N-use and resource allocation under N-limited conditions, and a minor but significant function in the later stages of senescence.
机译：在生理和分子水平上鉴定了玉米（Zea mays）衰老相关的豆荚蛋白基因See2beta，以确定其在衰老和资源分配中的作用。玉米突变体（Mu）群体的反向遗传学筛选确定了See2beta中的Mu插入。产生了用于插入纯合的玉米植物。这些See2突变和同级野生型植物在高或低量的氮（N）下生长。两种基因型的早期发育相似。然而，流苏尖端和衣领出现在突变体中发生得较早。突变体叶片的衰老遵循与野生型叶片相似的模式，但是在后来的采样点，突变体植物比野生型植物含有更多的叶绿素，并且光合活性显示出较小的扩展。野生型植物的总重量高于突变型植物，并且存在基因型x N相互作用。与相同处理下的野生型植物相比，低氮下的突变植物保持了穗轴重量。在转座子诱变的基础上得出结论，See2beta在氮有限的条件下，在氮的使用和资源分配中具有重要作用，在衰老的后期具有次要但重要的功能。

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