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首页> 外文期刊>The New Phytologist >Overexpression of wound-responsive RNA-binding proteins induces leaf senescence and hypersensitive-like cell death.
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Overexpression of wound-responsive RNA-binding proteins induces leaf senescence and hypersensitive-like cell death.

机译：伤口反应性RNA结合蛋白的过表达诱导叶片衰老和超敏性细胞死亡。

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Leaf senescence is a form of programmed cell death, and involves regulated expression of a specific set of senescence-associated genes (SAGs). In Arabidopsis, three UBA2 genes, UBA2a, UBA2b, and UBA2c, encode heterogeneous nuclear ribonucleoprotein (hnRNP)-type RNA-binding proteins. Previously, it has been demonstrated that expression of UBA2 genes is induced by mechanical wounding in a splice variant-dependent manner. Constitutive overexpression of the UBA2 genes proved lethal. Accordingly, a conditional gain-of-function system was used here to assess phenotypes related to UBA2 overexpression. Overexpression of each of the three UBA2 genes leads to a leaf yellowing/cell death-like phenotype in Arabidopsis plants. Expression levels of a number of SAGs, such as SAG13, SAG14, SAG15, SAG101, WRKY6, WRKY53, WRKY70, ACS2, ACS6, CML38 and SIRK, were elevated upon induction of UBA2 overexpression, as were transcripts of multiple wounding- and defense-related genes, including EDS1, CK1, JR1, WR3 and MPK3. Elevated ethylene biosynthesis and hypersensitive-like patterns of cell death and callose deposition, shown by Trypan blue and aniline blue staining, respectively, were also observed following induced overexpression of UBA2a, UBA2b, and UBA2c. These results indicate that induction of UBA2 gene expression stimulates leaf yellowing and cell death phenotypes through senescence and defense response pathways.
机译：叶片衰老是程序性细胞死亡的一种形式，涉及特定的一组衰老相关基因（SAG）的调控表达。在拟南芥中，三个UBA2基因UBA2a，UBA2b和UBA2c编码异种核糖核蛋白（hnRNP）型RNA结合蛋白。以前，已经证明UBA2基因的表达是通过剪接变体依赖性方式的机械损伤诱导的。 UBA2基因的组成型过表达被证明具有致命性。因此，此处使用条件功能获得系统来评估与UBA2过表达相关的表型。在拟南芥植物中，三个UBA2基因的每一个的过表达导致叶片变黄/细胞死亡样表型。在诱导UBA2过表达后，许多SAG的表达水平升高，例如SAG13，SAG14，SAG15，SAG101，WRKY6，WRKY53，WRKY70，ACS2，ACS6，CML38和SIRK，以及多处损伤和防御的转录本相关基因，包括EDS1，CK1，JR1，WR3和MPK3。在诱导过表达UBA2a，UBA2b和UBA2c后，还分别观察到了锥虫蓝和苯胺蓝染色显示的乙烯生物合成升高和细胞死亡和call质沉积的超敏样模式。这些结果表明，UBA2基因表达的诱导通过衰老和防御反应途径刺激叶片变黄和细胞死亡表型。

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