...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The New Phytologist >Breeding with rare defective alleles (BRDA): a natural Populus nigra HCT mutant with modified lignin as a case study
【24h】

Breeding with rare defective alleles (BRDA): a natural Populus nigra HCT mutant with modified lignin as a case study

机译：稀有缺陷等位基因（BRDA）的繁殖：以改良的木质素为原料的天然黑杨HCT突变体

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Next-generation (NG) sequencing in a natural population of Populus nigra revealed a mutant with a premature stop codon in the gene encoding hydroxycinnamoyl-CoA: shiki-mate hydroxycinnamoyl transferasel (HCT1), an essential enzyme in lignin biosynthesis. The lignin composition of P. nigra trees homozygous for the defective allele was compared with that of heterozygous trees and trees without the defective allele. The lignin was characterized by phenolic profiling, lignin oligomer sequencing, thioacidolysis and NMR. In addition, HCT1 was heterologously expressed for activity assays and crosses were made to introduce the mutation in different genetic backgrounds. HCT1 converts p-coumaroyl-CoA into p-coumaroyl shikimate. The mutant allele, PnHCTI-A73,encodes a truncated protein, and trees homozygous for this recessive allele have a modified lignin composition characterized by a 17-fold increase in p-hydroxyphenyl units. Using the lignin pathway as proof of concept, we illustrated that the capture ofrare defective alleles is a straightforward approach to initiate reverse genetics and accelerate tree breeding. The proposed breeding strategy, called 'breeding with rare defective alleles' (BRDA), should be widely applicable, independent of the target gene or the species.
机译：在黑杨的自然种群中进行的下一代（NG）测序揭示了一个突变体，该突变体在编码羟肉桂酰基-CoA的基因中具有过早的终止密码子：sh草酸羟肉桂酰基转移酶（HCT1），这是木质素生物合成中必不可少的酶。比较了有缺陷等位基因纯合子的黑黑杨的木质素组成与没有杂合等位基因的杂合树和木质素的比较。木质素的特征在于酚分析，木质素低聚物测序，硫代酸解和NMR。此外，HCT1被异源表达用于活性测定，并进行杂交以将突变引入不同的遗传背景。 HCT1将对香豆酰辅酶A转化为对香豆酰sh草酸酯。突变型等位基因PnHCTI-A73编码截短的蛋白，该隐性等位基因纯合子的树木具有修饰的木质素组成，其特征是对羟基苯单位增加了17倍。使用木质素途径作为概念的证明，我们说明了捕获罕见缺陷等位基因是启动反向遗传学和加速树木育种的直接方法。拟议的育种策略，即“稀有缺陷等位基因育种”（BRDA），应广泛适用，而与靶基因或物种无关。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号