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首页> 外文期刊>The Journal of Reproduction and Development >Triploid human embryonic stem cells derived from tripronuclear zygotes displayed pluripotency and trophoblast differentiation ability similar to the diploid human embryonic stem cells
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Triploid human embryonic stem cells derived from tripronuclear zygotes displayed pluripotency and trophoblast differentiation ability similar to the diploid human embryonic stem cells

机译：来自三核合子的三倍体人类胚胎干细胞显示出与二倍体人类胚胎干细胞相似的多能性和滋养细胞分化能力

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摘要

Because the diploid human embryonic stem cells (hESCs) can be successfully derived from tripronuclear zygotes thus, they can serve as an alternative source of derivation of normal karyotype hESC lines. The aim of the present study was to compare the pluripotency and trophoblast differentiation ability of hESCs derived from tripronuclear zygotes and diploid hESCs. In the present study, a total of 20 tripronuclear zygotes were cultured; 8 zygotes developed to the blastocyst stage and 1 hESC line was generated. Unlike the previous studies, chromosomal correction of tripronuclear zygotes during derivation of hESCs did not occur. The established line carries 3 sets of chromosomes and showed a numerical aberration. Although the cell line displayed an abnormal chromosome number, it was found the cell line has been shown to be pluripotent with the ability to differentiate into 3 embryonic germ layers both in vitro and in vivo. The expression of X inactive specific transcript (XIST) in mid-passage (passage 42) of undifferentiated triploid hESCs was detected, indicating X chromosome inactivation of the cell line. Moreover, when this cell line was induced to differentiate toward the trophoblast lineage, morphological and functional trophoblast cells were observed, similar to the diploid hESC line.
机译：因为二倍体人类胚胎干细胞（hESCs）可以成功地来源于三核合子，所以它们可以作为正常核型hESC系的另一种来源。本研究的目的是比较源自三核合子和二倍体hESC的hESC的多能性和滋养细胞分化能力。在本研究中，共培养了20个三核合子。 8个受精卵发育到了胚泡期，并产生了1个hESC系。与以前的研究不同，在hESCs衍生过程中未进行三核合子的染色体校正。所建立的品系携带3组染色体，并显示出数字像差。尽管该细胞系显示出异常的染色体数，但发现该细胞系具有多能性，能够在体外和体内分化为3个胚芽层。检测到未分化的三倍体hESC的中间传代（传代42）中X失活的特异性转录本（XIST）的表达，表明该细胞系的X染色体失活。此外，当诱导该细胞系向滋养层谱系分化时，观察到形态和功能的滋养层细胞，类似于二倍体hESC系。

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