Detection of breast cancer cells in ductal lavage fluid by methylation-specific PCR.

Evron E; Dooley WC; Umbricht CB; Rosenthal D; Sacchi N; Gabrielson E; Soito AB; Hung DT; Ljung B; Davidson NE; Sukumar S

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Detection of breast cancer cells in ductal lavage fluid by methylation-specific PCR.

机译：通过甲基化特异性PCR检测导管灌洗液中的乳腺癌细胞。

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If detected early, breast cancer is curable. We tested cells collected from the breast ducts by methylation-specific PCR (MSP). Methylated alleles of Cyclin D2, RAR-beta, and Twist genes were frequently detected in fluid from mammary ducts containing endoscopically visualised carcinomas (17 cases of 20), and ductal carcinoma in situ (two of seven), but rarely in ductal lavage fluid from healthy ducts (five of 45). Two of the women with healthy mammograms whose ductal lavage fluid contained methylated markers and cytologically abnormal cells were subsequently diagnosed with breast cancer. Carrying out MSP in these fluid samples may provide a sensitive and powerful addition to mammographic screening for early detection of breast cancer.

机译：如果及早发现，乳腺癌是可以治愈的。我们通过甲基化特异性PCR（MSP）测试了从乳腺导管收集的细胞。 Cyclin D2，RAR-beta和Twist基因的甲基化等位基因经常在含有内窥镜下可见癌的乳腺导管（20例中有17例）和原位导管癌（7例中的2例）的乳腺导管中发现，但在健康的导管（45条中的5条）。随后，有两名乳房X线照片健康的女性，其导管灌洗液中含有甲基化标记和细胞学异常细胞，随后被诊断出患有乳腺癌。在这些液体样本中执行MSP可以为乳腺钼靶筛查提供灵敏而有力的补充，以早期发现乳腺癌。

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来源
《The Lancet》 |2001年第9265期|共2页
作者
Evron E; Dooley WC; Umbricht CB; Rosenthal D; Sacchi N; Gabrielson E; Soito AB; Hung DT; Ljung B; Davidson NE; Sukumar S;
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正文语种 eng
中图分类医药、卫生;
关键词
Breast Neoplasms; DNA Methylation; Polymerase Chain Reaction; 乳腺肿瘤; DNA甲基化; 聚合酶链反应;

机译：Breast Neoplasms;DNA Methylation;Polymerase Chain Reaction;乳腺肿瘤;DNA甲基化;聚合酶链反应;

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专利

1. Detection of breast cancer cells in ductal lavage fluid by methylation-specific PCR. [J] . Evron E, Dooley WC, Umbricht CB, The Lancet . 2001,第9265期

机译：通过甲基化特异性PCR检测导管灌洗液中的乳腺癌细胞。
2. Patterns of reduced nipple aspirate fluid production and ductal lavage cellularity in women at high risk for breast cancer [J] . Susan A Higgins, Ellen T Matloff, David L Rimm, Breast Cancer Research . 2005,第6期

机译：罹患乳腺癌高风险女性的乳头抽吸液体减少和导管灌洗细胞减少的模式
3. Ductal lavage for detection of cellular atypia in women at high risk for breast cancer. [J] . Dooley WC, Ljung BM, Veronesi U, Journal of the National Cancer Institute . 2001,第21期

机译：导管灌洗法用于检测罹患乳腺癌高风险女性的细胞异型性。
4. Ductal Lavage in Women at High Risk for Breast Cancer [C] . International Multidisciplinary Congress . 2003

机译：乳腺癌高风险的妇女的导管灌洗
5. Gene regulation by estrogen and tamoxifen in estrogen-receptor positive and estrogen-receptor negative human breast cancer cells: Characterization and quantification by real-time PCR. [D] . Yuan, Xia. 2004

机译：雌激素受体阳性和雌激素受体阴性人类乳腺癌细胞中雌激素和他莫昔芬的基因调控：实时PCR的表征和定量。
6. A direct comparative study of methylation-specific PCR in ductal lavage fluid breast cancer tissue normal breast parenchyma and plasma in women with early breast cancer [O] . GPH Gui, D Twelves, A Nerurkar, 2009

机译：对早期乳腺癌女性的导管灌洗液乳腺癌组织正常乳腺实质和血浆中甲基化特异性PCR的直接比较研究
7. A direct comparative study of methylation-specific PCR in ductal lavage fluid, breast cancer tissue, normal breast parenchyma and plasma in women with early breast cancer [O] . GPH Gui, D Twelves, A Nerurkar, 2009

机译：对早期乳腺癌女性的导管灌洗液，乳腺癌组织，正常乳腺实质和血浆中甲基化特异性PCR的直接比较研究
8. Early Detection of Breast Cancer by Molecular Analysis of Ductal Lavage Fluid [R] . Sukumar, S. 2004

机译：乳管浸润液分子分析早期检测乳腺癌

Detection of breast cancer cells in ductal lavage fluid by methylation-specific PCR.

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