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首页> 外文期刊>The journals of gerontology.Series A. Biological sciences and medical sciences >Pathways change in expression during replicative aging in Saccharomyces cerevisiae.
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Pathways change in expression during replicative aging in Saccharomyces cerevisiae.

机译：酿酒酵母复制衰老过程中表达途径的改变。

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摘要

Yeast replicative aging is a process resembling replicative aging in mammalian cells. During aging, wild-type haploid yeast cells enlarge, become sterile, and undergo nucleolar enlargement and fragmentation; we sought gene expression changes during the time of these phenotypic changes. Gene expression studied via microarrays and quantitative real-time reverse-transcription polymerase chain reaction (qPCR) has shown reproducible, statistically significant changes in messenger RNA (mRNA) of genes at 12 and 18-20 generations. Our findings support previously described changes towards aerobic metabolism, decreased ribosome gene expression, and a partial environmental stress response. Our findings include a pseudostationary phase, downregulation of methylation-related metabolism, increased nucleotide excision repair-related mRNA, and a strong upregulation of many of the regulatory subunits of protein phosphatase I (Glc7). These findings are correlated with aging changes in higher organisms as well as with the known involvement of protein phosphorylation states during yeast aging.
机译：酵母复制性衰老是类似于哺乳动物细胞中复制性衰老的过程。在衰老过程中，野生型单倍体酵母细胞会增大，变得无菌，并经历核仁增大和断裂;我们寻求在这些表型变化期间的基因表达变化。通过微阵列和定量实时逆转录聚合酶链反应（qPCR）研究的基因表达已显示12和18-20代基因的信使RNA（mRNA）具有可重现的，统计学上显着的变化。我们的发现支持先前描述的有氧代谢改变，核糖体基因表达降低和部分环境应激反应。我们的发现包括伪平稳期，甲基化相关代谢的下调，核苷酸切除修复相关mRNA的增加以及蛋白磷酸酶I（Glc7）的许多调节亚基的强烈上调。这些发现与高等生物的衰老变化以及酵母衰老过程中蛋白质磷酸化状态的已知参与有关。

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