The role of telomere attrition in limiting the replicative capacity of cells in culture is well established. In humans, epidemiologic evidence suggests telomere length (TL) in leukocytes is highly variable at birth and inversely related to age. Although calorie restriction (CR) significantly increases life span in most rodent models, its association with TL is unknown. Using linear regression analysis, TLs (as measured by Southern blot analysis) of skeletal muscle (a postmitotic tissue that largely represents early development TL), fat, leukocytes, and skin were tested for effects of age, sex, and diet in 48 control and 23 calorie restriction rhesus monkeys. After controlling for the individual's muscle mean TL, differences between leukocytes muscle and skin muscle were significantly associated with age (p = .002; p = .002) and sex (p = .003; p = .042), but not calorie restriction (p = .884; p = .766). Despite an age-dependent shortening of TL in leukocytes and skin, calorie restriction did not significantly affect TL dynamics in these samples.
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机译：端粒磨损在限制细胞在培养物中的复制能力中的作用已得到充分确立。在人类中，流行病学证据表明白细胞中的端粒长度（TL）在出生时高度可变，并且与年龄成反比。尽管卡路里限制（CR）在大多数啮齿动物模型中可显着延长其寿命，但与TL的关联尚不清楚。使用线性回归分析，在48个对照组和对照组中测试了骨骼肌（主要代表早期发育TL的有丝分裂后组织），脂肪，白细胞和皮肤的TL（通过Southern blot分析测得）对年龄，性别和饮食的影响。 23卡路里限制恒河猴。在控制了个体的肌肉平均TL之后，白细胞肌肉和皮肤肌肉之间的差异与年龄（p = .002; p = .002）和性别（p = .003; p = .042）显着相关，但与卡路里限制无关（p = .884; p = .766）。尽管白细胞和皮肤中的TL随年龄而变短，但热量限制并没有显着影响这些样品中的TL动态。
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