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首页> 外文期刊>The Journal of rheumatology >Cathepsin K in aseptic hip prosthesis loosening: expression in osteoclasts without polyethylene wear particles.
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Cathepsin K in aseptic hip prosthesis loosening: expression in osteoclasts without polyethylene wear particles.

机译：组织蛋白酶K在无菌髋关节假体中松动：在破骨细胞中表达，无聚乙烯磨损颗粒。

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OBJECTIVE: To investigate the expression of the bone matrix degrading cysteine proteinase cathepsin K and to determine the colocalization of cathepsin K with polyethylene (PE) particles in tissue specimens of patients with aseptic hip prosthesis loosening (AHPL). METHODS: The expression of cathepsin K was studied by immunohistochemistry in tissue specimens of 9 patients with aseptically loosened acetabular components of failed cementless total hip replacements. The expression of cathepsin K was compared to that of the macrophage marker CD68 by serial section analysis. Double labeling of the expression of cathepsin K or CD68 by immunohistochemistry and of PE particles by modified Oil Red staining method was performed. RESULTS: Cathepsin K could be predominantly detected in osteoclasts attached to the bone tissue, while only a few (CD68+) mononuclear and multinucleated foreign body giant cells (MGC) were positive for this enzyme. By double labeling with Oil Red staining we found the majority of CD68 positive cells of the periprosthetic tissue that were colocalized with PE particles. However, cathepsin K-expressing osteoclasts could not be stained with Oil Red. CONCLUSION: The present data suggest that in AHPL neither mononuclear cells nor MGC but rather osteoclasts are mainly involved in cathepsin K mediated bone matrix destruction. Using double labeling of immunohistochemistry and Oil Red staining we observed that the cathepsin K-expressing osteoclasts did not include PE particles.
机译：目的：探讨降解半胱氨酸蛋白酶组织蛋白酶K的骨基质的表达，并测定组织蛋白酶K与聚乙烯（PE）颗粒在无菌性髋关节假体松动（AHPL）患者组织样本中的共定位。方法：采用免疫组织化学方法对9例因非骨水泥全髋置换失败而无菌性髋臼组件松动的患者的组织标本进行组织蛋白酶K的表达研究。通过连续切片分析将组织蛋白酶K的表达与巨噬细胞标记CD68的表达进行比较。通过免疫组织化学法对组织蛋白酶K或CD68的表达进行双重标记，并通过改良的油红染色法对PE颗粒进行双重标记。结果：组织蛋白酶K主要在附着于骨组织的破骨细胞中被检测到，而只有少数（CD68 +）单核和多核异物巨细胞（MGC）对该酶呈阳性。通过油红染色的双重标记，我们发现假体周围组织的大多数CD68阳性细胞与PE颗粒共定位。但是，表达组织蛋白酶K的破骨细胞不能用油红染色。结论：目前的数据表明，在AHPL中，单核细胞和MGC都没有，破骨细胞主要与组织蛋白酶K介导的骨基质破坏有关。使用免疫组织化学和油红染色的双重标记，我们观察到表达组织蛋白酶K的破骨细胞不包含PE颗粒。

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