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Distinction of Cladonia rei and C. subulata based on molecular, chemical and morphological characteristics

机译:基于分子,化学和形态学特征对Cladonia rei和C. subulata进行区分

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摘要

Cladonia rei and Cladonia subulata are morphologically similar, but chemically different cup lichens of dry grasslands and nutrient-poor ruderal habitats. Recently, C. rei has been synonymized with C. subulata on the basis of combined morphological and chemical investigations. However, doubts remained due to a molecular divergent North American sample of C. rei compared to European C. subulata. To clarify the situation, using molecular methods, we analysed chemically different European samples of C. rei and C. subulata, as well as other morphologically or chemically similar Cladonia species. Molecular data show that European and North American samples of C. rei belong to the same clade, which is closely related to C. fimbriata and followed by a subclade with C. coniocraea and C. ochrochlora. The subclade of C. subulata appears to be distinct from C. rei. In concordance with molecular data, the presence of homosekikaic acid is the determining chemical feature for C. rei. In addition, C. humilis and C. innominata proved to be molecularly distinct species.
机译:Cladonia rei和Cladonia subulata在形态上相似,但化学上在干燥的草原和营养不良的生境中呈杯状地衣。最近,在形态学和化学研究相结合的基础上,C。rei被称为C. subulata。但是,与北美的C. subulata相比,北美的C. rei样品在分子上存在差异,因此仍存在疑问。为了澄清这种情况,我们使用分子方法分析了欧洲不同物种的C. rei和C. subulata,以及其他形态或化学相似的Cladonia物种。分子数据显示,欧洲和北美的雷氏梭菌样本属于同一进化枝,与纤毛梭菌密切相关,其次是锥壳隐孢子虫和鳞衣藻。 C. subulata的子小节似乎与C. rei不同。与分子数据一致,高芥酸的存在是决定C. rei的化学特征。此外,H。humilis和C. innominata被证明是分子上不同的物种。

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