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首页> 外文期刊>The Laryngoscope: A Medical Journal for Clinical and Research Contributions in Otolaryngology, Head and Neck Medicine and Surgery, Facial Plastic and Reconstructive Surgery .. >Treatment effects of rhBMP-2 on invasiveness of oral carcinoma cell lines.
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Treatment effects of rhBMP-2 on invasiveness of oral carcinoma cell lines.

机译：rhBMP-2对口腔癌细胞株侵袭性的治疗作用。

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摘要

OBJECTIVE: To determine if recombinant human bone morphogenetic protein-2 (rhBMP-2) has biological effects on the invasiveness of human oral squamous cell carcinoma (OSCCA) cell lines. STUDY DESIGN: Laboratory investigation using six human OSCCA cell lines, with three cell lines having baseline gene expression of BMP-2 and three cell lines without baseline gene expression of BMP-2. METHODS: The invasiveness of each cell line was measured using a matrigel invasion assay with or without stimulation by rhBMP-2. A tumor metastasis quantitative PCR array was used to establish whether observed findings from the invasion assay correlated to changes in gene expression. RESULTS: There was a significant increase in tumor cell invasion in response to rhBMP-2 in all BMP-2 positive cell lines but no change in the cell lines that did not express the BMP-2 gene. Quantitative PCR revealed that changes in gene expression were distinctly different based on the baseline gene expression of BMP-2 and favored a more metastatic genotype in the BMP-2-positive cells. CONCLUSIONS: Recombinant human BMP-2 has an adverse biological effect on invasiveness of human OSCCA cell lines in vitro. This adverse effect is dependent on the baseline gene expression of BMP-2. Changes in expression of genes involved with tumor metastasis correlated to the invasion assay findings. These data raise concern for the safe application of rhBMP-2 for reconstruction of bone defects in oral cancer patients.
机译：目的：确定重组人骨形态发生蛋白2（rhBMP-2）是否对人口腔鳞状细胞癌（OSCCA）细胞的侵袭具有生物学影响。研究设计：使用六个人OSCCA细胞系进行实验室研究，其中三个具有BMP-2基线基因表达的细胞系和三个没有BMP-2基线基因表达的细胞系。方法：使用matrigel入侵测定法，在有或没有rhBMP-2刺激的情况下，测量每个细胞系的侵袭性。肿瘤转移定量PCR阵列用于确定从入侵试验中观察到的发现是否与基因表达的变化相关。结果：在所有BMP-2阳性细胞系中，对rhBMP-2的应答，肿瘤细胞侵袭均显着增加，但不表达BMP-2基因的细胞系无变化。定量PCR显示，基于BMP-2的基线基因表达，基因表达的变化明显不同，并且倾向于BMP-2阳性细胞中更具转移性的基因型。结论：重组人BMP-2对人OSCCA细胞体外侵袭具有不利的生物学作用。这种不良反应取决于BMP-2的基线基因表达。与肿瘤转移有关的基因表达的变化与侵袭试验的发现有关。这些数据引起人们对rhBMP-2在口腔癌患者中重建骨缺损的安全应用的关注。

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