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首页> 外文期刊>The Laryngoscope: A Medical Journal for Clinical and Research Contributions in Otolaryngology, Head and Neck Medicine and Surgery, Facial Plastic and Reconstructive Surgery .. >Evidence of mast cell activity in the middle ears of children with otitis media with effusion.
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Evidence of mast cell activity in the middle ears of children with otitis media with effusion.

机译：有积液的中耳炎患儿中耳肥大细胞活动的证据。

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OBJECTIVES: This is the first study to report the presence of tryptase, a reflection of mast cell activity, in chronic middle ear effusion of patients whose atopic status was characterized. DESIGN AND METHODS: Mediator activity of mast cells and eosinophils was measured prospectively from effusion of 33 randomly selected patients and 5 control subjects with chronic otitis media with effusion (OME). Atopy was determined by enzyme-linked immunosorbent assay. Middle ear biopsies from a second group of 8 OME patients and 4 controls were fixed in plastic and stained immunohistochemically for mast cells. RESULTS: Sixty-one percent of patients had extensive activation of mast cells in their middle ears. Among those with elevated tryptase in their effusion, 95.6% were atopic and 94.7% also had elevated levels of effusion eosinophilic cationic protein (ECP). Tryptase levels were elevated only in the effusion of atopic patients, as compared with 5 controls (P < .01). Mast cells were present in 6 of 8 OME ears and absent in all 4 normal ears. CONCLUSION: Mast cells and its mediator tryptase, both indicators of a Th2-driven immune response, are present in a majority of ears that have chronic effusion. These findings support the hypothesis that middle ear mucosa is capable of an allergic response and that the inflammation within the middle ear of most OME patients is allergic in nature.
机译：目的：这是第一个报告在特应性状态患者的慢性中耳积液中存在类胰蛋白酶（肥大细胞活性的一种反映）的研究。设计与方法：前瞻性地从33例随机选择的患者和5例患有慢性中耳炎伴渗出液（OME）的渗出液中测量肥大细胞和嗜酸性粒细胞的介体活性。通过酶联免疫吸附测定法确定特应性。将来自第二组的8名OME患者和4名对照的中耳活检标本固定在塑料中，并对肥大细胞进行免疫组织化学染色。结果：61％的患者中耳肥大细胞被广泛激活。在其分泌物中类胰蛋白酶升高的患者中，异位性过敏的比例为95.6％，嗜酸性粒细胞阳离子蛋白（ECP）的升高的比例也为94.7％。与5名对照相比，仅在特应性患者的渗出液中类胰蛋白酶水平升高（P <.01）。肥大细胞存在于8个OME耳朵中的6个而在所有4个正常耳朵中都不存在。结论：肥大细胞及其介体类胰蛋白酶是Th2驱动的免疫反应的两个指标，存在于大多数具有慢性积液的耳朵中。这些发现支持了以下假设：中耳粘膜具有过敏反应能力，而大多数OME患者中耳内部的炎症本质上是过敏性的。

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