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Extracranial drainage of cerebrospinal fluid: a study of beta-transferrins in nasal and lymphatic tissues.

机译:脑脊液颅外引流:鼻和淋巴组织中β-转铁蛋白的研究。

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摘要

OBJECTIVE: To determine whether an alternative route of cerebrospinal fluid (CSF) drainage exists through the nasal mucosa and the cervical lymphatic system. STUDY DESIGN: A prospective study was carried out on 18 patients at a university teaching hospital. METHODS: Ten patients undergoing routine endoscopic sinus surgeries and eight patients undergoing neck dissections were recruited for this study. Tissues were sampled from the middle turbinate, nasopharynx, and upper septum in the first group; jugulodigastric lymph nodes and nasopharyngeal tissues were obtained from the second group. Specimens were subjected to immunofixation electrophoresis in an attempt to identify the presence of beta-1 and beta-2 transferrins. Serum samples were obtained from each subject to serve as controls. RESULTS: All tissue specimens contained beta-1 transferrin; none showed evidence of beta-2 transferrin. CONCLUSION: Using this technique, an alternate route of CSF drainage through the nose and the cervical lymphatic system could not be confirmed. Nevertheless, a new technique of performing immunofixation in solid tissues for the purpose of beta-transferrin identification is described.
机译:目的:确定是否存在通过鼻粘膜和宫颈淋巴系统排泄脑脊液的另一种途径。研究设计:在一家大学教学医院对18例患者进行了一项前瞻性研究。方法:本研究招募了10例行常规内镜鼻窦手术的患者和8例行颈清扫术的患者。在第一组的中鼻甲,鼻咽和上隔中取样组织。从第二组中获得了胃食管的淋巴结和鼻咽组织。对标本进行免疫固定电泳,以鉴定是否存在β-1和β2转铁蛋白。从每个受试者获得血清样品作为对照。结果:所有组织标本均含有β-1转铁蛋白。没有人显示出β-2转铁蛋白的证据。结论:使用该技术无法确定通过鼻子和子宫颈淋巴系统引流脑脊液的另一种途径。然而,描述了一种用于β-转铁蛋白鉴定的在实体组织中进行免疫固定的新技术。

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