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首页> 外文期刊>The Laryngoscope: A Medical Journal for Clinical and Research Contributions in Otolaryngology, Head and Neck Medicine and Surgery, Facial Plastic and Reconstructive Surgery .. >Expression of a cathelicidin antimicrobial peptide is augmented in cholesteatoma.
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Expression of a cathelicidin antimicrobial peptide is augmented in cholesteatoma.

机译：cathelicidin抗菌肽在胆脂瘤中的表达增加。

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OBJECTIVES/HYPOTHESIS: Antimicrobial peptides are active defense components of innate immunity. Their importance was confirmed at epithelial surfaces as immediate barrier effectors in preventing infection. Cathelicidins are peptide antibiotics that are receiving increasing attention. Several studies have shown that overexpression of cathelicidin results in augmented protection against bacterial infection and prevention of local infection and systemic invasion of microbes. The goal of the study was to investigate whether cathelicidin is upregulated in cholesteatoma epithelium compared with normal skin. STUDY DESIGN: Twenty patients from a prospective study of cholesteatoma tissues and normal skins were enrolled in the study. The specimens were divided into two portions. One portion was used for subsequent RNA studies; the other was used for immunohistochemical staining. METHODS: Reverse transcriptase-polymerase chain reaction was used to assess the expression levels of cathelicidin messenger RNA (mRNA) both in cholesteatoma and in normal skin. Presumptive concentration of cathelicidin mRNA and beta2-microglobulin mRNA was evaluated. Ratio of beta2-microglobulin to cathelicidin was analyzed in each group. The expressions of cathelicidin in cholesteatoma and normal skin epithelium were investigated by an immunohistochemical technique. RESULTS: Cathelicidin mRNA in cholesteatoma epithelium was increased 5.5-fold compared with normal skin of the ear canal. In cholesteatoma epithelium, cathelicidin was located in all the layers, but in the normal skin it was expressed only in the granular and prickle cell layers. CONCLUSIONS: Cathelicidin is augmented in cholesteatoma epithelium, and the data in the present study are in agreement with the hypothesis that cathelicidin is likely to act as a key component in the first line of defense at the surface epithelium.
机译：目的/假设：抗菌肽是先天免疫的主动防御成分。他们的重要性在上皮表面被确认为预防感染的直接屏障效应物。鞘磷脂是肽类抗生素，受到越来越多的关注。多项研究表明，cathelicidin的过度表达可增强对细菌感染的保护作用，并防止局部感染和微生物的系统入侵。该研究的目的是调查胆脂瘤上皮中的cathelicidin与正常皮肤相比是否上调。研究设计：来自胆脂瘤组织和正常皮肤的前瞻性研究的20名患者入选了该研究。标本分为两部分。一部分用于随后的RNA研究;另一部分用于后续RNA研究。另一个用于免疫组织化学染色。方法：采用逆转录酶-聚合酶链反应评估胆脂瘤和正常皮肤中cathelicidin信使RNA（mRNA）的表达水平。估计cathelicidin mRNA和β2-微球蛋白mRNA的浓度。在每组中分析了β2-微球蛋白与cathelicidin的比率。免疫组化技术检测胆脂瘤和正常皮肤上皮中cathelicidin的表达。结果：胆脂瘤上皮中的Cathelicidin mRNA与正常耳道皮肤相比增加了5.5倍。在胆脂瘤上皮中，cathelicidin位于所有层中，但在正常皮肤中，它仅在颗粒细胞和痒细胞层中表达。结论：胆脂瘤上皮中增加了Cathelicidin，并且本研究中的数据与以下假设相吻合：Cathelicidin可能是表面上皮第一道防线中的关键组成部分。

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