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首页> 外文期刊>The Laryngoscope: A Medical Journal for Clinical and Research Contributions in Otolaryngology, Head and Neck Medicine and Surgery, Facial Plastic and Reconstructive Surgery .. >Detection of herpesvirus DNAs in perilymph obtained from patients with sensorineural hearing loss by real-time polymerase chain reaction.
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Detection of herpesvirus DNAs in perilymph obtained from patients with sensorineural hearing loss by real-time polymerase chain reaction.

机译：实时聚合酶链反应检测感官神经性听力损失患者外周淋巴中的疱疹病毒DNA。

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OBJECTIVES/HYPOTHESIS: Perilymph and peripheral blood mononuclear cells (PBMCs) from patients with bilateral severe sensorineural hearing loss (SNHL) were evaluated for the presence of DNA from cytomegalovirus (CMV), herpes simplex virus (HSV), and human herpesvirus (HHV)6. STUDY DESIGN: A prospective clinical study. METHODS: The subjects were 14 patients who underwent cochlear implantation and 1 patient who underwent gentamicin injection in the inner ear. We attempted to detect viral DNA from perilymph and PBMCs by real-time polymerase chain reaction (rtPCR). RESULTS: CMV DNA was detected in two perilymph specimens obtained from patients who were diagnosed as congenitally symptomatic CMV infection, although no CMV DNA was detected in PBMCs. Neither HSV DNA nor HHV6 DNA was detected in any other perilymph specimens. CMV DNA was detected in three PBMC samples, HSV DNA was detected in two samples, and HHV6 DNA was detected in six samples. CONCLUSION: CMV may persistently infect the inner ear of patients with congenital CMV infection, and rtPCR analysis may prove to be a valuable tool for investigating the etiology of SNHL.
机译：目的/假设：对双侧严重感觉神经性听力损失（SNHL）患者的外周血和外周血单核细胞（PBMC）进行了巨细胞病毒（CMV），单纯疱疹病毒（HSV）和人疱疹病毒（HHV）DNA的评估。 6。研究设计：前瞻性临床研究。方法：对象为14例接受耳蜗植入的患者和1例接受庆大霉素内耳注射的患者。我们尝试通过实时聚合酶链反应（rtPCR）从周淋巴和PBMC中检测病毒DNA。结果：尽管从PBMC中未检测到CMV DNA，但在被诊断为先天性症状性CMV感染的患者的两个淋巴标本中检测到CMV DNA。在其他任何淋巴标本中均未检测到HSV DNA和HHV6 DNA。在三个PBMC样品中检测到CMV DNA，在两个样品中检测到HSV DNA，在六个样品中检测到HHV6 DNA。结论：CMV可能会持续感染先天性CMV感染患者的内耳，而rtPCR分析可能是研究SNHL病因的有价值的工具。

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