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首页> 外文期刊>The Laryngoscope: A Medical Journal for Clinical and Research Contributions in Otolaryngology, Head and Neck Medicine and Surgery, Facial Plastic and Reconstructive Surgery .. >Injection of human mesenchymal stem cells improves healing of vocal folds after scar excision--a xenograft analysis.
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Injection of human mesenchymal stem cells improves healing of vocal folds after scar excision--a xenograft analysis.

机译：人间充质干细胞的注射改善了疤痕切除后声带的愈合-异种移植分析。

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OBJECTIVES: Using a xenograft model the aim was to analyze if injection of human mesenchymal stem cells (hMSC) into the rabbit vocal fold (VF), after excision of an established scar, can improve the functional healing of the VF. STUDY DESIGN: Prospective design with an experimental xenograft model. METHODS: The VFs of 12 New Zealand rabbits were injured by a bilateral localized resection. After 9 weeks the scar after the resection was excised and hMSC were injected into the VFs. After another 10 weeks 10 VFs were dissected and stained for histology. Lamina propria thickness and relative content of collagen type I were measured. Viscoelasticity of 14 VFs at phonatory frequencies was quantified by a simple-shear rheometer. The hMSC survival was determined using a human DNA specific reference probe, that is, FISH analysis. RESULTS: The viscoelastic measurements, that is, dynamic viscosity and elastic shear modulus for the hMSC-treated VFs, were found to be similar to those of normal controls and were significantly lower than those of untreated controls (P < .05). A significant reduction in lamina propria thickness was also shown for the hMSC treated VFs compared with the untreated VFs (P < .05). This histologic finding corresponded with the viscoelastic results. No hMSC survived 10 weeks after the injection. CONCLUSIONS: Human mesenchymal stem cells injected into the rabbit VF following the excision of a chronic scar, were found to enhance the functional healing of the VF with reduced lamina propria thickness and restored viscoelastic shear properties.
机译：目的：使用异种移植模型，目的是分析在切除已确定的疤痕后，将人间充质干细胞（hMSC）注入兔声带（VF）是否可以改善VF的功能愈合。研究设计：采用实验异种移植模型的前瞻性设计。方法：12只新西兰兔的VFs通过双侧局部切除术受伤。 9周后，切除切除后的疤痕并将hMSC注射到VF中。再过10周后，将10个VF解剖并进行组织学染色。测量固有层的厚度和I型胶原的相对含量。通过简单剪切流变仪对在发声频率下14 VF的粘弹性进行了定量。使用人类DNA特异性参考探针（即FISH分析）确定hMSC的存活率。结果：hMSC处理的VF的粘弹性测量值，即动态粘度和弹性剪切模量，与正常对照组相似，并且显着低于未处理对照组（P <.05）。经hMSC处理的VF与未处理的VF相比，固有层厚度也显着减少（P <.05）。该组织学发现与粘弹性结果相对应。注射后10周，没有hMSC存活。结论：切除慢性疤痕后，将人间充质干细胞注射入兔VF中，可增强VF的功能性愈合，减少固有层厚度并恢复粘弹性剪切特性。

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