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首页> 外文期刊>The Laryngoscope: A Medical Journal for Clinical and Research Contributions in Otolaryngology, Head and Neck Medicine and Surgery, Facial Plastic and Reconstructive Surgery .. >Interaction of cochlear nucleus explants with semiconductor materials.
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Interaction of cochlear nucleus explants with semiconductor materials.

机译：耳蜗核外植体与半导体材料的相互作用。

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OBJECTIVE/HYPOTHESIS: Implantable hearing devices such as cochlear implants and auditory brainstem implants deliver auditory information through electrical stimulation of auditory neurons. The combination of microelectronic electrodes with auditory nerve cells may lead to further improvement of the hearing quality with these devices. Whereas several kinds of neurons are known to grow on semiconductor substrates, interactions of cochlear nucleus (CN) neurons with such materials have yet to be described. MATERIALS AND METHODS: To investigate survival and growth behavior of CN neurons on different semiconductor materials. CN explants from postnatal day 10 Sprague-Dawley rats were cultured for 96 hours in Neurobasal medium on polished and unpolished silicon wafers (p-type Si [100] and p-type Si3N4[100]) as well as plastic surface. These surfaces had been coated with poly-L-lysine and laminin. Neuronal outgrowth was examined using image analysis software after immunohistologic staining for neurofilament. Neurite length and directional changes were quantified. Additionally, neurite morphology and adhesion to the semiconductor material was evaluated by scanning electron microscopy. RESULTS: Although proper adhesion of CN explants was seen, no neurite growth could be detected on unpolished silicon wafers (Si and Si3N4). Compared with the other test conditions, polished, laminin-coated Si3N4 wafers showed best biocompatibility regarding neurite length and number per explant. CN explants developed a mean of eight neurons with an average length of 236 mum in 96 hours of culture on these wafers. CONCLUSION: The results of this study demonstrate the general possibility of CN neuron growth in culture on semiconductors in vitro. The differences in neuron length and number per explant indicate that the growth of CN neurons is influenced by the semiconductor substrate as well as extracellular matrix proteins, with laminin-coated p-type Si3N4[100] being a preferable material for future hybrid experiments on auditory-neuron semiconductor chips.
机译：目的/假设：可植入的听力装置，例如耳蜗植入物和听觉脑干植入物，通过电刺激听觉神经元传递听觉信息。微电子电极与听神经细胞的结合可导致这些设备的听力质量进一步提高。尽管已知几种神经元在半导体衬底上生长，但是耳蜗核（CN）神经元与此类材料的相互作用尚待描述。材料与方法：研究CN神经元在不同半导体材料上的存活和生长行为。出生后第10天的Sprague-Dawley大鼠的CN外植体在Neurobasal培养基上在抛光和未抛光的硅晶片（p型Si [100]和p型Si3N4 [100]）以及塑料表面上培养96小时。这些表面已经用聚-L-赖氨酸和层粘连蛋白包被。免疫组织学染色后，用图像分析软件检查神经元长出的神经丝。定量神经突长度和方向变化。另外，通过扫描电子显微镜评价神经突的形态和对半导体材料的粘附。结果：尽管看到了CN外植体的适当粘附，但在未抛光的硅晶片（Si和Si3N4）上未检测到神经突生长。与其他测试条件相比，抛光，层粘连蛋白涂覆的Si3N4晶圆在神经突长度和每个外植体数量方面表现出最佳的生物相容性。在这些圆片上培养96小时后，CN外植体平均产生了8个神经元，平均长度为236微米。结论：本研究结果证明了在体外培养的半导体中CN神经元生长的一般可能性。神经元长度和每外植体数量的差异表明，CN神经元的生长受半导体底物以及细胞外基质蛋白的影响，层粘连蛋白包被的p型Si3N4 [100]是将来在听觉上进行杂交实验的理想材料。 -中子半导体芯片。

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