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首页> 外文期刊>The Laryngoscope: A Medical Journal for Clinical and Research Contributions in Otolaryngology, Head and Neck Medicine and Surgery, Facial Plastic and Reconstructive Surgery .. >Confocal scanning laser microscopy evidence of biofilms in patients with chronic rhinosinusitis.
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Confocal scanning laser microscopy evidence of biofilms in patients with chronic rhinosinusitis.

机译：共聚焦扫描激光显微镜检查证明慢性鼻鼻窦炎患者体内存在生物膜。

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OBJECTIVES: The recent detection of bacterial biofilms on the sinus mucosa of patients with chronic rhinosinusitis (CRS) has implicated biofilms in the pathogenesis of this condition. Electron microscopy has been the main modality used to document the presence of biofilms on sinus tissue, however, it has inherent problems associated with tissue preparation and sampling. Recently, Confocal Scanning Laser Micrsocopy (CSLM) has emerged as a noninvasive, nondestructive technique for the analysis of biofilms. This study used CSLM as the means of investigating biofilm presence in CRS patients. STUDY DESIGN AND METHODS: A prospective study comparing the presence of bacterial biofilms on the sinus mucosa of CRS and control patients was conducted using CSLM. Thirty eight CRS patients undergoing endoscopic sinus surgery and nine control patients were enrolled in this study. Demographic and clinical information was recorded from each patient and intraoperatively, sinus culture specimens and mucosal samples were obtained for microbiologic and microscopic examination. RESULTS: Using previously documented CSLM criteria, bacterial biofilms were found in 17 (44%) of the 38 CRS patients. No biofilm structures were evident in any of the controls. Patients having undergone previous sinus surgery seemed to have a higher incidence of biofilms compared with the incidence for those undergoing their first procedure. The difference however was not statistically significant. No correlation between positive bacterial cultures and biofilm presence was observed. CONCLUSIONS: The CSLM detection of biofilms in CRS patients and their absence in controls further supports the hypothesis that biofilms may play a role in the pathogenesis of CRS. This study's lower reported incidence of biofilms compared with that of previous studies might reflect the increased accuracy of biofilm detection with CSLM.
机译：目的：最近在慢性鼻鼻窦炎（CRS）患者的鼻窦粘膜上发现细菌生物膜已将生物膜与这种病的发病机制相关。电子显微镜检查已成为记录鼻窦组织生物膜存在的主要方式，但是，它具有与组织制备和取样相关的固有问题。近来，共聚焦扫描激光显微镜（CSLM）已经成为一种用于生物膜分析的非侵入性，非破坏性技术。这项研究使用CSLM作为研究CRS患者中生物膜存在的方法。研究设计和方法：使用CSLM进行一项前瞻性研究，比较CRS和对照患者的鼻窦黏膜上细菌生物膜的存在。本研究纳入了38例接受内镜鼻窦手术的CRS患者和9例对照患者。记录每位患者的人口统计学和临床​​信息，并在术中获取鼻窦培养标本和粘膜标本进行微生物学和显微镜检查。结果：使用先前记录的CSLM标准，在38例CRS患者中的17例（44％）中发现了细菌生物膜。在任何对照中都没有明显的生物膜结构。与接受首次手术的患者相比，先前接受过鼻窦手术的患者似乎具有更高的生物膜发生率。然而，差异在统计学上不显着。没有观察到阳性细菌培养物和生物膜存在之间的相关性。结论：CSLM检测CRS患者中的生物膜及其在对照中的缺失，进一步支持了生物膜可能在CRS的发病机理中起作用的假设。与先前的研究相比，该研究报告的生物膜发生率较低，这可能反映了CSLM检测生物膜的准确性提高。

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