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首页> 外文期刊>The Laryngoscope: A Medical Journal for Clinical and Research Contributions in Otolaryngology, Head and Neck Medicine and Surgery, Facial Plastic and Reconstructive Surgery .. >Acoustic trauma augments the cochlear immune response to antigen.
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Acoustic trauma augments the cochlear immune response to antigen.

机译：声学损伤会增强耳蜗对抗原的免疫反应。

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OBJECTIVES/HYPOTHESIS: To test whether noise-exposure, which activates a cochlear immune response with cytokine expression and infiltration of circulating leukocytes could augment the response to antigen (Ag). STUDY DESIGN: Randomized, prospective, mice. METHODS: We sensitized mice to an Ag, injected it intrathecally, and subsequently exposed the mice to noise (8-16 kHz, 90, 100, or 118 dB for 2 hours). Control mice received either noise exposure alone (100 or 118 dB), Ag challenge alone, intrathecal surgery and phosphate-buffered saline injection or no treatment. Four hours or 7 days later the mice were killed and cochlear sections were evaluated immunohistochemically for CD45, ICAM-1, and phospho-nuclear transcription factor-kappaB expression. RESULTS: Intrathecal Ag injection caused no hearing loss, but did result in a small immune response. Loud noise (118 dB) caused severe hearing loss and slight inflammation. The number of CD45-positive cells was significantly greater in the Ag plus-118 dB noise group relative to the Ag-alone group or 118 dB noise-exposure group. ICAM expression was seen in the lower part of the spiral ligament and small vessels within the normal cochlea. The amount of expression increased after Ag injection and acoustic trauma. Activated nuclear transcription factor-kappaB occurred in the nuclei of hair cells, supporting cells, spiral ligament fibrocytes, and neurons 4 hours after noise exposure. CONCLUSIONS: It seems that noise exposure can activate a cochlear immune response, which in the presence of Ag, allows for greater recruitment of inflammatory cells than occurred in response to Ag alone.
机译：目的/假设：为了测试噪声暴露是否能激活耳蜗免疫应答并激活细胞因子表达并循环白细胞浸润，从而增强对抗原（Ag）的应答。研究设计：随机，前瞻性，小鼠。方法：我们使小鼠对Ag敏感，将其鞘内注射，然后将小鼠暴露于噪声下（8-16 kHz，90、100或118 dB 2小时）。对照小鼠仅接受噪声暴露（100或118 dB），仅接受Ag攻击，鞘内手术和磷酸盐缓冲盐水注射或不接受任何治疗。 4小时或7天后，处死小鼠，并用免疫组织化学方法评估CD45，ICAM-1和磷酸核转录因子-κB表达的耳蜗切片。结果：鞘内注射银没有引起听力损失，但确实导致了较小的免疫反应。大声的声音（118 dB）导致严重的听力损失和轻微的炎症。相对于单独的Ag组或118 dB噪声暴露组，Ag plus-118 dB噪声组中CD45阳性细胞的数量显着增加。正常耳蜗内的螺旋韧带下部和小血管中可见ICAM表达。注射银和听觉创伤后，表达量增加。噪声暴露后4小时，活化的核转录因子-κB发生在毛细胞，支持细胞，螺旋韧带纤维细胞和神经元的细胞核中。结论：似乎噪声暴露可以激活耳蜗免疫反应，在存在银的情况下，与单独对银的反应相比，炎症细胞的募集更大。

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