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首页> 外文期刊>The Journal of Physiology >Inhibition of alpha 7-containing nicotinic ACh receptors by muscarinic M1 ACh receptors in rat hippocampal CA1 interneurones in slices.
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Inhibition of alpha 7-containing nicotinic ACh receptors by muscarinic M1 ACh receptors in rat hippocampal CA1 interneurones in slices.

机译：切片中大鼠海马CA1间神经元中毒蕈碱M1 ACh受体对含α7烟碱ACh受体的抑制作用。

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Cys-loop ligand-gated nicotinic ACh receptors (nAChRs) and G protein-coupled muscarinic ACh receptors (mAChRs) are expressed on rat hippocampal interneurones where they can regulate excitability, synaptic communication and cognitive function. Even though both nAChRs and mAChRs appear to co-localize to the same interneurones, it is not clear whether there is crosstalk between them. We utilized patch-clamp techniques to investigate this issue in rat hippocampal CA1 interneurones in slices under conditions where synaptic transmission was blocked. The alpha7 nAChR-mediated currents were activated by choline, and when the activation of this receptor was preceded by the activation of the M(1) mAChR subtype, the amplitude of alpha7 responses was significantly reduced in a rapidly reversible and voltage-independent manner, without any change in the kinetics of responses. This M(1) mAChR-mediated inhibition of alpha7 nAChRs was through a PLC-, calcium- and PKC-dependent signal transduction cascade. These data show that M(1) mAChRs and alpha7 nAChRs are functionally co-localized on individual rat hippocampal interneurones where the activation of these particular mAChRs inhibits alpha7 nAChR function. This information will help to understand how these cholinergic receptor systems might be regulating neuronal excitability in the hippocampus in a manner that has relevance for synaptic plasticity and cognition.
机译：半胱氨酸环配体门控烟碱型ACh受体（nAChRs）和G蛋白偶联毒蕈碱型ACh受体（mAChRs）在大鼠海马中间神经元上表达，它们可以调节兴奋性，突触通讯和认知功能。即使nAChR和mAChR似乎共同定位在相同的中间神经元上，也不清楚它们之间是否存在串扰。我们利用膜片钳技术在突触传递受阻的条件下研究大鼠海马CA1中间神经元在切片中的这一问题。 alpha7 nAChR介导的电流被胆碱激活，并且在激活该受体之前先激活M（1）mAChR亚型，以快速可逆且独立于电压的方式显着降低了alpha7响应的幅度，响应动力学没有任何变化。这种M（1）mAChR介导的对alpha7 nAChRs的抑制作用是通过PLC，钙和PKC依赖性信号转导级联来实现的。这些数据显示M（1）mAChRs和alpha7 nAChRs在功能上共定位于单个大鼠海马interneurones，其中这些特定mAChRs的激活抑制了alpha7 nAChR功能。这些信息将有助于了解这些胆碱能受体系统可能如何以与突触可塑性和认知相关的方式调节海马神经元兴奋性。

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