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首页> 外文期刊>The Journal of Physiology >cAMP-independent responses of olfactory neurons in Xenopus laevis tadpoles and their projection onto olfactory bulb neurons.
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cAMP-independent responses of olfactory neurons in Xenopus laevis tadpoles and their projection onto olfactory bulb neurons.

机译：非洲爪蟾中嗅神经元的cAMP依赖性反应及其在嗅球神经元中的投射。

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We report on responses of olfactory receptor neurons (ORNs) upon application of amino acids and forskolin using a novel slice preparation of the olfactory epithelium of Xenopus laevis tadpoles. Responses were measured using the patch-clamp technique. Both amino acids and forskolin proved to be potent stimuli. Interestingly, a number of ORNs that responded to amino acids did not respond to forskolin. This suggests that some amino acids activate transduction pathways other than the well-known cAMP-mediated one. The differential processing of cAMP-mediated stimuli on the one hand and amino acid stimuli on the other was further elucidated by calcium-imaging of olfactory bulb neurons using a novel nose-olfactory bulb preparation of Xenopus laevis tadpoles. The projection pattern of amino acid-sensitive ORNs to olfactory bulb neurons differed markedly from the projection pattern of forskolin-sensitive ORNs. Olfactory bulb neurons activated by amino acids were located laterally compared to those activated by forskolin, and only a small proportion responded to both stimuli. The ensemble of neurons activated by forskolin was also activated by the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX) and the membrane-permeant cAMP analogue 8-(4-chlorophenylthio)adenosine 3',5'-cyclic monophosphate (pCPT-cAMP). We therefore conclude that sensory transduction of a number of amino acids is cAMP independent, and amino acid- and cAMP-mediated responses are processed differentially at the level of the olfactory bulb.
机译：我们报告了嗅觉受体神经元（ORNs）的应用氨基酸和毛喉动物使用非洲爪蟾t嗅上皮的新型切片制备后的反应。使用膜片钳技术测量反应。氨基酸和毛喉素都被证明是有效的刺激物。有趣的是，许多对氨基酸有反应的ORN对福司可林没有反应。这表明除了众所周知的cAMP介导的氨基酸外，某些氨基酸还激活了转导途径。使用非洲爪蟾eno的新型鼻嗅球制剂，通过嗅球神经元的钙成像，进一步阐明了一方面cAMP介导的刺激与氨基酸刺激的差异处理。氨基酸敏感的ORN对嗅球神经元的投影模式与毛喉素敏感的ORN的投影模式显着不同。与被毛喉素激活的嗅球神经元相比，被氨基酸激活的嗅球神经元位于侧面，只有一小部分对两种刺激作出反应。磷酸二酯酶抑制剂3-异丁基-1-甲基黄嘌呤（IBMX）和膜渗透性cAMP类似物8-（4-氯苯硫基）腺苷3'，5'-环一磷酸酯（pCPT-营）。因此，我们得出结论，许多氨基酸的感觉转导是独立于cAMP的，并且氨基酸和cAMP介导的应答在嗅球的水平上被不同地处理。

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