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首页> 外文期刊>The Proceedings of the New Zealand Society of Animal Production >Measuring short periods of wool fibre growth using radiolabelling
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Measuring short periods of wool fibre growth using radiolabelling

机译：使用放射性标记法测量羊毛纤维的短期生长

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摘要

Brief periods cannot be measured with traditional autoradiographic methods on wool fibres because the labelled points overlap. A dose of [~(35)S]cysteine was injected into 24 sites across the side of five Lincoln ewes. After 48 hours a second injection was administered to one site on each ewe, followed by an injection into another site every two hours until all 24 sites had been labeled twice. Three days later all 24 sites were injected again. Following autoradiography the distance between radiolabelled points was traced using a digitizer (50 fibres per site). A linear increase in length between points 1 and 2 was observed between sites (r~2 = 0.920) and the regression equation showed the fibre increased in length by 29.2 μm per site (P < 0.01). However, a linear decrease in length was observed down (P < 0.001) but not along (P = 0.813) the midside over the period delineated by injections 1 and 3. Removing the effect of site gave growth rates that reflected the overall mean of 28.9 μm/hour for all sites. This method could be used to determine changes in growth rate over a few hours or the response to a growth factor, hormone or nutrient over a time course of several hours.
机译：由于标记的点重叠，因此无法使用传统的放射自显影方法对羊毛纤维进行短暂测量。将一剂[〜（35）S]半胱氨酸注射到五只林肯母羊侧面的24个部位。 48小时后，对每只母羊的一个部位进行第二次注射，然后每两小时注射一次到另一个部位，直到所有24个部位都标记了两次。三天后，再次注射所有24个部位。放射自显影后，使用数字化仪（每个位点50条光纤）追踪放射性标记点之间的距离。在站点之间观察到点1和2之间的长度线性增加（r〜2 = 0.920），回归方程显示每个站点的纤维长度增加了29.2μm（P <0.01）。但是，在注射1和注射3所描绘的时间段内，观察到长度的线性下降（P <0.001），而不是沿着中侧（P = 0.813）。去除部位的影响后，生长速率反映出整体平均值为28.9。所有站点的微米/小时。该方法可用于确定几个小时内生长速率的变化或几个小时内对生长因子，激素或营养素的反应。

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