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A comparison of triploid induction validation techniques

机译：三倍体诱导验证技术的比较

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摘要

Using 13 diploid and triploid sunshine bass (white bass (Morone chrysops) female X striped bass (M. saxatilis) male) as a model, the most common triploidy verification techniques were cross-validated. Techniques compared where: DNA staining and fluorescence quantification with a flow cytometer, erythrocyte nuclear volume with a Coulter counter particle size analyser, silver staining of nucleolar organizer regions (NORs), and cytological karyotyping. Results indicated that the electronic techniquesof particle size analysis and flow cytometry were the simplest and quickest methods of validation. The major drawback of both these methods is the cost of the equipment required for analysis. Cytological karyotyping was the most accurate method for determining polyploidy because actual chromosome numbers were determined. It was also the most time-consuming and tedious. Silver staining was the least expensive technique, but NORs were sometimes difficult to detect and there were conflicting results in older fish.
机译：以13种二倍体和三倍体阳光鲈鱼（白鲈（Morone chrysops）雌性X条纹鲈鱼（M. saxatilis）雄性）为模型，对最常见的三倍体验证技术进行了交叉验证。比较了以下技术：使用流式细胞仪进行的DNA染色和荧光定量分析，使用Coulter计数器粒度分析仪的红细胞核体积，核仁组织区（NORs）的银染色以及细胞核型分析。结果表明，粒度分析和流式细胞术的电子技术是最简单，最快的验证方法。这两种方法的主要缺点是分析所需的设备成本。细胞核型分析是确定多倍体性的最准确方法，因为确定了实际的染色体数。这也是最耗时又乏味的。银染是最便宜的技术，但NORs有时难以检测，并且在较老的鱼中有相互矛盾的结果。

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