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HORMONE-INDUCED MULTIPLE SPAWNING OF CAPTIVE NASSAU GROUPER BROODSTOCK

机译:激素诱导的圈养NASSAU石斑鱼种群多发

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During a 5-month period from 3 December 1991 to 28 April 1992, two female Nassau grouper Epinephelus striatus were spawned three rimes and one female two times by hormone induction. The interval between spawnings for an individual female ranged from 28-75 d. Successful spawnings (fertilization rate > 50%) were induced in females with average oocyte diameters ranging from 517-544 mu m by a primary injection of human chorionic gonadotropin (HCG; 1,000 IU/kg body weight), followed by a second injection (500 IU/kg body weight) after 24 h. Time of strip-spawning ranged from 37-45 h after the first injection and was inversely related to average water temperature (range, 22.4-26.4 degrees C). Mean diameters spawned eggs were 871-959 mu m, and the number of eggs spawned per female ranged from 200,000 to 2,000,000. Fertilization and hatching success were 17.9-80.6% and 68.3-90.1%, respectively. The results demonstrate a reproductive capacity that minimizes broodstock requirements and enhances suitability of the Nassau grouper for aquaculture.
机译:在1991年12月3日至1992年4月28日的5个月中,通过激素诱导产生了两只雌性拿骚石斑鱼Epinephelus striatus,产下了三只子,一只雌性产了两次。雌性个体产卵间隔为28-75 d。初次注射人绒毛膜促性腺激素(HCG; 1,000 IU / kg体重),然后再次注射(500卵),在平均卵母细胞直径为517-544微米的雌性中成功产卵(受精率> 50%)。 IU / kg体重)在24小时后。第一次注入后条带产卵的时间为37-45小时,与平均水温(范围22.4-26.4摄氏度)成反比。产卵的平均直径为871-959微米,每只雌性产卵的数量为200,000至2,000,000。受精和孵化成功率分别为17.9-80.6%和68.3-90.1%。结果表明,繁殖能力可最大程度地减少亲鱼需求,并增强拿骚石斑鱼对水产养殖的适应性。

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