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首页> 外文期刊>The protein journal >Biochemical Characterization of a S-glutathionylated Carbonic Anhydrase Isolated from Gills of the Antarctic Icefish Chionodraco hamatus.
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Biochemical Characterization of a S-glutathionylated Carbonic Anhydrase Isolated from Gills of the Antarctic Icefish Chionodraco hamatus.

机译：从南极冰鱼Chionodraco hamatus的s中分离出的S-谷胱甘肽化的碳酸酐酶的生化特性。

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摘要

Gill cytoplasmic carbonic anhydrase of the haemoglobinless Antarctic icefish Chionodraco hamatus (Ice-CA) was directly sequenced and consists in 259 residues with an acetylated N-terminus. The molecular mass, deduced from the sequence, was 28.45 kDa, while mass spectrometry analysis of the native protein gave higher values. Treatment with dithiothreitol abolished this difference, indicating possible post-translational modifications. Isoelectric focusing analysis of Ice-CA suggested S-thiolation, which was identified as S-glutathionylation by immunostaining. Deglutathionylated Ice-CA maintained the anhydrase activity but showed higher susceptibility to hydrogen peroxide, suggesting that glutathione binding to Cys residues may have a role in the defence against oxidative damage. Ice-CA is characterized by lower thermal stability, higher activity and lower activation energy than its homologue gill CA of the temperate European eel, confirming the adaptation of the catalytic capacity to low temperatures. Alignment of Ice-CA with homologous enzymes from other fish shows high identity; the enzyme is grouped with a previously described fish CA monophyletic clade although Ice-CA shows several characteristics that can increase protein-solvent interaction and structural flexibility.
机译：直接对无血红蛋白的南极冰鱼Chionodraco hamatus（Ice-CA）的细胞质碳酸酐酶进行测序，该序列由259个残基组成，带有一个乙酰化的N末端。从该序列推导的分子量为28.45 kDa，而对天然蛋白质的质谱分析给出了更高的值。用二硫苏糖醇治疗消除了这种差异，表明可能存在翻译后修饰。 Ice-CA的等电聚焦分析表明存在S-巯基化作用，通过免疫染色鉴定为S-谷胱甘肽化。去谷胱甘肽化的Ice-CA保持了脱水酶的活性，但对过氧化氢的敏感性更高，表明谷胱甘肽与Cys残基结合可能在防御氧化损伤中起作用。 Ice-CA的特征是与欧洲温带鳗鱼的同源gCA相比，具有较低的热稳定性，较高的活性和较低的活化能，从而证实了催化能力对低温的适应性。 Ice-CA与其他鱼类的同源酶比对显示出很高的一致性;尽管Ice-CA显示出一些可增加蛋白质与溶剂相互作用和结构柔韧性的特征，但该酶与先前描述的鱼类CA单系进化枝分组。

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