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首页> 外文期刊>The Journal of Experimental Biology >NA+ AND CA2+ HOMEOSTATIC MECHANISMS IN ISOLATED CHLORIDE CELLS OF THE TELEOST OREOCHROMIS MOSSAMBICUS ANALYSED BY CONFOCAL LASER SCANNING MICROSCOPY
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NA+ AND CA2+ HOMEOSTATIC MECHANISMS IN ISOLATED CHLORIDE CELLS OF THE TELEOST OREOCHROMIS MOSSAMBICUS ANALYSED BY CONFOCAL LASER SCANNING MICROSCOPY

机译：共聚焦激光扫描显微镜分析最远古猿莫桑比克孤立氯化物细胞中NA +和CA2 +的稳态机制

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摘要

Chloride cells (CCs; recognised by the vital mitochondrial stain DASPEI) and pavement cells (PCs) isolated from tilapia opercular epithelium were adhered to Cell-Tak-coated glass coverslips and loaded with fluorescent probes for the measurement of intracellular concentrations of Na+ or Ca2+. Basal levels of cytosolic Na+ and Ca2+ ranged from 6.4 to 16.5 mmoll(-1) and from 76 to 110 nmoll(-1), respectively, and did not differ between CCs and PCs. In CCs, inhibition of Na+/K+-ATPase by ouabain or Cu2+ increased intracellular [Na+]. Replacing extracellular Na+ with N-methyl-D-glucamine(+) led to a rise in cytosolic [Ca2+] that was dependent on the extracellular [Ca2+], indicating that a Na+/Ca2+ exchanger was operating in reverse mode (importing Ca2+). The forward mode of this exchanger could be demonstrated by inhibition with bepridil. The CC has various pathways for passive Na+ influx: a tetrodotoxin-sensitive pathway, an amiloride-sensitive pathway and other as yet unidentified pathways. [References: 62]
机译：从罗非鱼眼皮上皮中分离出的氯化物细胞（CC;被线粒体重要染料DASPEI识别）和铺面细胞（PC）粘附在涂有Cell-Tak的玻璃盖玻片上，并装有荧光探针以测量细胞内Na +或Ca2 +的浓度。胞质Na +和Ca2 +的基础水平分别为6.4至16.5 mmoll（-1）和76至110 nmoll（-1），并且CC和PC之间没有差异。在CC中，哇巴因或Cu2 +对Na + / K + -ATPase的抑制作用会增加细胞内[Na +]。用N-甲基-D-葡糖胺（+）代替细胞外Na +导致依赖于细胞外[Ca2 +]的胞浆[Ca2 +]升高，这表明Na + / Ca2 +交换剂以反向模式运行（导入Ca2 +）。该交换剂的正向模式可以通过用苯普地尔抑制来证明。 CC具有多种被动Na +流入途径：河豚毒素敏感途径，阿米洛利敏感途径以及其他尚未确定的途径。 [参考：62]

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