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首页> 外文期刊>The Journal of Experimental Biology >Variation in salinity tolerance, gill Na+/K+-ATPase, Na+/K+/2Cl(-) cotransporter and mitochondria-rich cell distribution in three salmonids Salvelinus namaycush, Salvelinus fontinalis and Salmo salar
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Variation in salinity tolerance, gill Na+/K+-ATPase, Na+/K+/2Cl(-) cotransporter and mitochondria-rich cell distribution in three salmonids Salvelinus namaycush, Salvelinus fontinalis and Salmo salar

机译：三种鲑鱼Salvelinus namaycush，Salvelinus fontinalis和Salmo salar的耐盐性，g Na + / K + -ATPase，Na + / K + / 2Cl（-）共转运蛋白和线粒体富集细胞分布的变化

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摘要

We compared seawater tolerance, gill Na+/K+-ATPase and Na+/K+/2Cl(-) cotransporter (NKCC) abundance, and mitochondria-rich cell (MRC) morphology of three salmonids, lake trout Salvelinus namaycush, brook trout Salvelinus fontinalis and Atlantic salmon Salmo salar. They were transferred directly from 0 p.p.t. (parts per thousand; freshwater) to 30 p.p.t. seawater, or transferred gradually from 0 to 10, 20 and 30 p.p.t. at 1-week intervals and kept in 30 p.p.t. for 3 weeks. The survival rates of lake trout, brook trout and Atlantic salmon were 80%, 50% and 100% following direct transfer, and 80%, 100% and 100% during gradual transfer, respectively. Plasma Na+, K+ and Cl- concentrations in surviving lake trout increased rapidly and remained at high levels in 30 p.p.t. of both direct and gradual transfer, whereas those in brook trout showed a transient increase following direct transfer but did not change significantly during gradual transfer. Only minor changes in plasma ions were observed in Atlantic salmon smolts in both direct and gradual transfer. These results suggest that lake trout retains some degree of euryhalinity and that brook trout possesses intermediate euryhalinity between lake trout and Atlantic salmon smolts. Gill Na+/K+-ATPase activity of lake trout and brook trout increased in seawater, whereas that of Atlantic salmon smolts was already upregulated in freshwater and remained high after seawater exposure. NKCC abundance was upregulated in parallel with gill Na+/K+-ATPase activity in each species. Immunocytochemistry with anti-Na+/K+-ATPase alpha-subunit and anti-NKCC revealed that the two ion transporters were colocalized on the basolateral membrane of gill MRCs. Immunopositive MRCs were distributed on both primary filaments and secondary lamellae in all three species kept in freshwater; following transfer to seawater this pattern did not change in lake trout and brook trout but lamellar MRCs disappeared in Atlantic salmon. Previous studies on several teleost species have suggested that filament and lamellar MRCs would be involved in seawater and freshwater acclimation, respectively. However, our results in lake trout and brook trout suggest that lamellar MRCs could be also functional during seawater acclimation.
机译：我们比较了三种鲑鱼，湖鳟Salvelinus namaycush，na鳟Salvelinus fontinalis和大西洋的耐海水性，g Na + / K + -ATPase和Na + / K + / 2Cl（-）共转运蛋白（NKCC）丰度以及线粒体富集细胞（MRC）的形态。鲑鱼Salmo salar。他们是从下午0点直接转移过来的。 （千分之一;淡水）至30 p.p.t.海水，或从零到10、20和30 p.p.t.每隔1周一次并保持在30 p.p.t. 3个星期。直接转移后，湖鳟，溪鳟和大西洋鲑的成活率分别为80％，50％和100％，在逐渐转移过程中分别为80％，100％和100％。尚存的湖鳟的血浆Na +，K +和Cl-浓度迅速增加并在30 p.p.t保持高水平。直接和渐进转移的数量，而在鳟鱼中的那些在直接转移后显示出短暂的增加，但在逐渐转移过程中没有显着变化。在直接转移和逐步转移中，在大西洋鲑鱼中仅观察到血浆离子的微小变化。这些结果表明，湖鳟鱼具有一定程度的鱼腥感，溪鳟鱼在湖鳟鱼和大西洋鲑鱼之间具有中等的鱼腥感。海水中湖鳟和溪鳟的Na + / K + -ATPase活性增加，而大西洋鲑鱼的mol在淡水中已经被上调，并且在暴露于海水后仍保持较高水平。在每种物种中，NKCC的丰度与g Na + / K + -ATPase活性同时上调。用抗Na + / K + -ATPaseα-亚基和抗NKCC进行的免疫细胞化学分析显示，这两种离子转运蛋白共定位于g MRC的基底外侧膜上。免疫阳性的MRCs分布在淡水中所有三个物种的初级细丝和次级薄片上。转移到海水中后，湖鳟和溪鳟的形态没有变化，但大西洋鲑的层状MRC消失了。先前对几种硬骨鱼类的研究表明，长丝和层状MRC分别参与海水和淡水适应。然而，我们在湖鳟和溪鳟的研究结果表明，层状MRCs在海水适应过程中也可能起作用。

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