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首页> 外文期刊>The Journal of Experimental Biology >Induced cell proliferation in putative haematopoietic tissues of the sea star, Asterias rubens (L.).
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Induced cell proliferation in putative haematopoietic tissues of the sea star, Asterias rubens (L.).

机译：在海星Asterias rubens（L.）的假定造血组织中诱导细胞增殖。

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The coelomic fluid of the echinoderm Asterias rubens possesses large populations of circulating coelomocytes. This study aimed to expand the knowledge about the haematopoietic sources of these cells. Injection of the immune-stimulating molecules lipopolysaccharide (LPS) and concanavalin A (ConA) resulted in an increase in coelomocytes. To investigate if these molecules induce cell proliferation in putative haematopoietic tissues (HPTs), short-term exposure of the substitute nucleotide 5-bromo-2'-deoxyuridine (BrdU) was conducted. Immunohistochemical analysis, using fluorescein-labelled antibodies to trace BrdU, showed pronounced cell division in the coelomic epithelium and axial organ. In the pyloric caeca, not considered as an HPT, proliferation was not detected. BrdU labelling of monolayers of cells obtained by collagenase treatment of coelomic epithelium, axial organ and Tiedemann body revealed induced cell proliferation in response to both LPS and ConA while proliferation of pyloric caeca and circulating coelomocytes remained sparse. By using confocal microscopy it was observed that both the morphology and functional behaviour of cells released from explants of coelomic epithelium showed high similarity to those of circulating phagocytes. It was concluded that the increased coelomocyte numbers observed in response to LPS and ConA were reflected in an induced cell proliferation in coelomic epithelium, axial organ and Tiedemann body, which reinforces the idea that these organs are HPTs and the sources of coelomocyte renewal.
机译：棘皮动物天牛的体液具有大量循环中的内皮细胞。这项研究旨在扩大有关这些细胞的造血来源的知识。注射免疫刺激分子脂多糖（LPS）和伴刀豆球蛋白A（ConA）会导致内皮细胞增加。为了研究这些分子是否在假定的造血组织（HPT）中诱导细胞增殖，进行了替代核苷酸5-溴-2'-脱氧尿苷（BrdU）的短期暴露。免疫组织化学分析使用荧光素标记的抗体来追踪BrdU，显示在结肠上皮和轴向器官中明显的细胞分裂。在不被视为HPT的幽门盲肠中，未检测到增殖。通过胶原酶处理结肠上皮，轴器官和Tiedemann体获得的单层细胞BrdU标记显示，LPS和ConA均可引起诱导的细胞增殖，而幽门盲肠和循环性白细胞的增殖仍然很少。通过共聚焦显微镜观察，从腔上皮外植体释放的细胞的形态和功能行为均与循环吞噬细胞高度相似。结论是，响应LPS和ConA观察到的增加的白细胞数目反映在腔上皮，轴器官和Tiedemann体中诱导的细胞增殖中，这强化了这些器官是HPT和更新白细胞的来源的观点。

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