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首页> 外文期刊>The Journal of Experimental Biology >New methods for field collection of human skin volatiles and perspectives for their application in the chemical ecology of human-pathogen-vector interactions.
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New methods for field collection of human skin volatiles and perspectives for their application in the chemical ecology of human-pathogen-vector interactions.

机译：人体皮肤挥发物现场收集的新方法及其在人-病原体-载体相互作用的化学生态学中的应用前景。

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摘要

Odours emitted by human skin are of great interest to biologists in many fields, with practical applications in forensics, health diagnostic tools and the ecology of blood-sucking insect vectors of human disease. Convenient methods are required for sampling human skin volatiles under field conditions. We experimentally compared four modern methods for sampling skin odours: solvent extraction, headspace solid-phase micro-extraction (SPME), and two new techniques not previously used for the study of mammal volatiles, contact SPME and dynamic headspace with a chromatoprobe design. These methods were tested and compared both on European subjects under laboratory conditions and on young African subjects under field conditions. All four methods permitted effective trapping of skin odours, including the major known human skin volatile compounds. In both laboratory and field experiments, contact SPME, in which the time of collection was restricted to 3 min, provided results very similar to those obtained with classical headspace SPME, a method that requires 45 min of collection. Chromatoprobe sampling also proved to be very sensitive, rapid and convenient for the collection of human-produced volatiles in natural settings. Both contact SPME and chromatoprobe design may considerably facilitate the study of human skin volatiles under field conditions, opening new possibilities for examining the olfactory cues mediating the host-seeking behaviour of mosquito vectors implicated in the transmission of major diseases.
机译：在许多领域，生物学家对人类皮肤发出的气味非常感兴趣，它们在法医学，健康诊断工具和人类疾病的吸血昆虫媒介生态学中都有实际应用。在现场条件下，需要一种方便的方法来采样人的皮肤挥发物。我们通过实验比较了四种采样皮肤异味的方法：溶剂萃取，顶空固相微萃取（SPME）和以前未用于研究哺乳动物挥发物的两种新技术，即采用色谱探针设计的SPME和动态顶空接触技术。在实验室条件下的欧洲受试者和野外条件下的非洲青年受试者中测试并比较了这些方法。所有四种方法都可以有效捕获皮肤异味，包括主要的已知人类皮肤挥发性化合物。在实验室和现场实验中，联系SPME（将收集时间限制为3分钟）所提供的结果与经典顶空SPME所获得的结果非常相似，这种方法需要45分钟的收集时间。事实证明，色谱探针采样非常灵敏，快速且方便，可用于在自然环境中收集人为产生的挥发物。接触SPME和色谱探针的设计都可以极大地促进田间条件下人体皮肤挥发物的研究，从而为检查介导与主要疾病传播相关的蚊媒寻求宿主行为的嗅觉线索提供了新的可能性。

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