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首页> 外文期刊>The Journal of Experimental Biology >Mitochondrial uncoupling as a regulator of life-history trajectories in birds: an experimental study in the zebra finch
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Mitochondrial uncoupling as a regulator of life-history trajectories in birds: an experimental study in the zebra finch

机译：线粒体解偶联作为鸟类生活史轨迹的调节剂：斑马雀的实验研究

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Mitochondria have a fundamental role in the transduction of energy from food into ATP. The coupling between food oxidation and ATP production is never perfect, but may nevertheless be of evolutionary significance. The 'uncoupling to survive' hypothesis suggests that 'mild' mitochondrial uncoupling evolved as a protective mechanism against the excessive production of damaging reactive oxygen species (ROS). Because resource allocation and ROS production are thought to shape animal life histories, alternative life-history trajectories might be driven by individual variation in the degree of mitochondrial uncoupling. We tested this hypothesis in a small bird species, the zebra finch (Taeniopygia guttata), by treating adults with the artificial mitochondrial uncoupler 2,4-dinitrophenol (DNP) over a 32-month period. In agreement with our expectations, the uncoupling treatment increased metabolic rate. However, we found no evidence that treated birds enjoyed lower oxidative stress levels or greater survival rates, in contrast to previous results in other taxa. In vitro experiments revealed lower sensitivity of ROS production to DNP in mitochondria isolated from skeletal muscles of zebra finch than mouse. In addition, we found significant reductions in the number of eggs laid and in the inflammatory immune response in treated birds. Altogether, our data suggest that the 'uncoupling to survive' hypothesis may not be applicable for zebra finches, presumably because of lower effects of mitochondrial uncoupling on mitochondrial ROS production in birds than in mammals. Nevertheless, mitochondrial uncoupling appeared to be a potential life-history regulator of traits such as fecundity and immunity at adulthood, even with food supplied ad libitum
机译：线粒体在从食物到ATP的能量转换中具有基本作用。食物氧化和ATP产生之间的耦合从来都不是完美的，但是可能具有进化意义。 “解耦生存”假说表明，“轻度”线粒体解偶联的发展是一种保护性机制，可防止过度产生破坏性活性氧（ROS）。由于资源分配和活性氧生产被认为会影响动物的生活史，因此，其他生活史轨迹可能是由线粒体解偶联程度的个体差异所驱动。我们通过在32个月内用人工线粒体解偶联剂2,4-二硝基苯酚（DNP）处理成年动物，在小型鸟类斑马雀科（Taeniopygia guttata）中验证了该假设。与我们的预期相符，解偶联处理提高了代谢率。但是，我们没有证据表明经过处理的鸟类具有较低的氧化应激水平或较高的成活率，这与之前在其他分类单元中的结果相反。体外实验表明，从雀斑骨骼肌分离的线粒体中，ROS对DNP的敏感性低于小鼠。此外，我们发现处理过的禽类的产卵数和炎性免疫反应均明显减少。总而言之，我们的数据表明“解耦生存”假说可能不适用于斑马雀，大概是因为线粒体解偶联对鸟类线粒体ROS产生的影响要比哺乳动物低。然而，线粒体解偶联似乎是成年后诸如繁殖力和免疫力等潜在生活史调节因子，即使随意提供食物也是如此。

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