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首页> 外文期刊>The Journal of Experimental Biology >Lipid accumulation in the ovaries of a marine shrimp Penaeus semisulcatus (De haan)
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Lipid accumulation in the ovaries of a marine shrimp Penaeus semisulcatus (De haan)

机译：海洋虾对虾对虾（De haan）卵巢中的脂质积累

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By the end of oocyte development, the ovaries of Penaeus semisulcatus have accumulated almost equal amounts (approximately 16 mg lipid g super(-1) protein) of phospholipids and triacylglycerols. The phospholipids consist mainly of phosphatidylcholine (75-80%) and phosphatidylethanolamine (20-25%). Approximately 30% of the total fatty acid content of both phospholipids and triacylglycerols is made up of polyunsaturated fatty acids. In fractions obtained by centrifugation of ovarian homogenates, most of the increase in levels of ovarian lipids during ovarian maturation was associated with an increase in triacylglycerol levels in the floating fat fraction and of phospholipids in the infranatant fraction. The presence of polyunsaturated fatty acids in the ovaries indicates the occurrence of lipid transport to the ovary during oocyte maturation. The gradual decrease in the relative abundance of polyunsaturated fatty acids as the ovaries matured supports previously published results suggesting intra-ovarian synthesis of saturated and mono-unsaturated fatty acids. Most of the lipids found in the female haemolymph (64.8%) were recovered in the high-density lipoprotein fraction after density ultracentrifugation. The haemocyanin fraction recovered from this stage of fractionation contained substantial amounts of lipid (16.8%) that could be removed by further sequential centrifugation at a higher NaBr density, leaving less than 0.9% of the total haemolymph lipids associated with this fraction. While 16.2% of the lipids were recovered from the very high-density lipoprotein fractions, these lipoproteins carried only 64-89 mu g lipid mg super(-1) protein compared with 538.9 mu g lipid mg super(-1) protein in the high-density lipoprotein fraction, indicating that the high-density lipoproteins are more likely to be the main transporters of lipids to the ovary. However, the contribution of very high-density lipoproteins to lipid transport cannot be ruled out at this stage. In this study, we present two models for lipid transport to the ovary based on the abundance of phospholipids and triacylglycerols in the haemolymph and on the amounts of polyunsaturated fatty acids accumulated within the ovary during vitellogenesis.
机译：到卵母细胞发育结束时，半边对虾的卵巢已积聚了几乎相等量的磷脂和三酰基甘油（约16 mg脂质g super（-1）蛋白质）。磷脂主要由磷脂酰胆碱（75-80％）和磷脂酰乙醇胺（20-25％）组成。磷脂和三酰基甘油的总脂肪酸含量的大约30％由多不饱和脂肪酸组成。在通过离心卵巢匀浆而获得的馏分中，卵巢成熟过程中卵巢脂质水平的大部分增加与漂浮脂肪馏分中三酰基甘油水平的增加以及infranatant馏分中磷脂的增加有关。卵巢中多不饱和脂肪酸的存在表明在卵母细胞成熟过程中脂质转运到卵巢。随着卵巢成熟，多不饱和脂肪酸的相对丰度逐渐降低，这支持了先前发表的结果，表明卵巢内合成了饱和和单不饱和脂肪酸。密度超速离心后，雌性血淋巴中发现的大多数脂质（64.8％）在高密度脂蛋白组分中回收。从分馏的这一阶段回收的血蓝蛋白馏分包含大量的脂质（16.8％），可以通过在更高的NaBr密度下进一步进行连续离心来去除，从而留下的血脂比该馏分的总血淋巴脂质少0.9％。虽然从非常高密度的脂蛋白馏分中回收了16.2％的脂质，但这些脂蛋白仅携带64-89μg脂质mg super（-1）蛋白，而在高密度脂蛋白中只有538.9μg脂质mg super（-1）蛋白-高密度脂蛋白部分，表明高密度脂蛋白更可能是脂质向卵巢的主要转运蛋白。但是，目前尚不能排除非常高密度的脂蛋白对脂质转运的贡献。在这项研究中，我们基于血淋巴中磷脂和三酰甘油的丰度以及卵黄发生过程中卵巢内积累的多不饱和脂肪酸的数量，提出了两种脂质转运至卵巢的模型。

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