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首页> 外文期刊>The Journal of Experimental Biology >Effects of static magnetic fields on the development and aging of Caenorhabditis elegans
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Effects of static magnetic fields on the development and aging of Caenorhabditis elegans

机译：静磁场对秀丽隐杆线虫生长和衰老的影响

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摘要

The current study investigated the possible effects of static magnetic fields (SMFs) on the developmental and aging processes of Caenorhabditis elegans. Nematodes were grown in the presence of SMFs of strengths varying from 0 to 200 mT. The rate of development and the lifespan were recorded. Treatment with a 200 mT SMF reduced the development time from the L2 to the L3 stage by 20%, from L3 to L4 by 23%, and from L4 to young adult by 31%. After SMF treatment, the average lifespan was reduced from 31 days to 24 days for wild-type nematodes. The up-regulation of clk-1, lim-7, daf-2, unc-3 and age-1 after SMF treatment was verified by quantitative real-time RT-PCR. Apparently, induction of gene expression is selective and dose dependent. The total developmental time was significantly reduced for the lin-4, lin-14, lin-41 and lim-7 mutants, but not for the let-7, clk-1, unc-3 and age-1 mutants. Lifespan analyses revealed that the let-7, unc-3 and age-1 mutants were not affected by SMF treatment. Here we show that SMFs accelerate nematode development and shorten nematode lifespan through pathways associated with let-7, clk-1, unc-3 and age-1.
机译：当前的研究调查了静态磁场（SMF）对秀丽隐杆线虫的发育和衰老过程的可能影响。线虫在强度从0到200 mT不等的SMF存在下生长。记录发展速度和寿命。使用200 mT SMF进行治疗，使从L2到L3阶段的发育时间减少了20％，从L3到L4的发育时间减少了23％，从L4到年轻成年人的发育时间减少了31％。 SMF处理后，野生型线虫的平均寿命从31天减少到24天。通过定量实时RT-PCR验证了SMF处理后clk-1，lim-7，daf-2，unc-3和age-1的上调。显然，基因表达的诱导是选择性的并且是剂量依赖性的。 lin-4，lin-14，lin-41和lim-7突变体的总发育时间显着减少，而let-7，clk-1，unc-3和age-1突变体却没有。寿命分析显示，let-7，unc-3和age-1突变体不受SMF处理的影响。在这里，我们显示SMF通过与let-7，clk-1，unc-3和age-1相关的途径来加速线虫的发育并缩短线虫的寿命。

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